Dutta et al1 reported impressive results using universal primer 16S rRNA polymerase chain reaction (PCR) to diagnose sepsis among 242 patients in a level III neonatal intensive care unit (NICU). However, because 2 of 52 bacteremic patients (4%) had falsely negative results, DeCamp et al,2 in the accompanying commentary, were unenthusiastic, concluding that “universal PCR is still not accurate enough to justify withholding antibiotic therapy in neonates being evaluated for sepsis.”
Newman TB, Kohn MA. Universal Primer Polymerase Chain Reaction Looks Promising for Newborns With Low Pretest Probability of Sepsis. Arch Pediatr Adolesc Med. 2009;163(7):675–676. doi:10.1001/archpediatrics.2009.121
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