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May 1955

Serum Pigment Studies in Newborn Infants: I. Erythroblastosis Fetalis

Author Affiliations

From the Division of Pediatrics, University of Tennessee College of Medicine, the John Gaston Hospital and Le Bonheur Children's Hospital.

AMA Am J Dis Child. 1955;89(5):544-552. doi:10.1001/archpedi.1955.02050110658004

Serum pigments in infants with erythroblastosis fetalis have been studied spectrophotometrically by Abelson and Boggs1 and by Waters and co-workers.2 Recently Boggs and Abelson3 have determined alkaline hematin levels in erythroblastosis fetalis in an effort to evaluate the severity of the disease. A major difficulty associated with spectrophotometric studies of serum is that withdrawal of blood and separation of plasma or serum is practically always attended by hemolysis to some degree. Hemoglobin thus released may contribute significantly to light absorption, particularly in the region of 415 mμ. We have overcome this difficulty by separating the hemoglobin from other serum pigments by paper electrophoresis, following which spectrophotometric analyses have been done without the interference of hemoglobin.

ANALYTICAL METHODS  Multiple blood specimens were collected for analysis (a) from the umbilical vein at the time of delivery immediately after pulsation had ceased, (b) from the umbilical or femoral vein before,