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May 1955

Anionic Resin Measurement of Protein-Bound I131 in Euthyroid Children

Author Affiliations

San Francisco; Chapel Hill, N. C.; San Francisco
Bank of America-Giannini Foundation Research Fellow in Pediatrics, 1953-1954; present address: Department of Pediatrics, University of North Carolina School of Medicine (Dr. Winters).; From the Departments of Pediatrics and Radiology, University of California School of Medicine, the Radioisotope Unit of Veterans Administration Hospital, and the Children's Hospital, San Francisco.

AMA Am J Dis Child. 1955;89(5):572-574. doi:10.1001/archpedi.1955.02050110686008

The measurement of thyroid hormone in the blood has been done more frequently in recent years by several methods: the chemical determination of protein- or serum-precipitable iodine1; various measurements of conversion of inorganic to organic iodide, for instance, RBC-plasma ratio,2 using I131 as a tracer, and measurement of butanol-extractable iodine.3

We have reported4 the use of anionic resin for measurement of protein-bound iodine I131 in human adult plasma; in the present article we present measurements on euthyroid children. Patients are given I131, and at intervals thereafter blood is drawn. The plasma is then treated with anionic resin. Anionic resin adsorbs inorganic iodide from plasma, leaving organic iodine practically untouched. The measurement is done by scintillation crystal counting of the plasma after it is treated with resin and separated by centrifugation, the resin and supernatant plasma being counted separately. This procedure can be done