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By combining formal presentations on various facets of the subject of paper electrophoresis with informal and sometimes lengthy discussion, 22 of the world's pioneers in this field have provided the reader with a rather comprehensive review of this popular laboratory procedure. Detailed descriptions are given of the types of basic apparatus which have been employed and of the conditions under which electrophoretic separations are carried out. Emphasis is placed upon the importance of interpreting results of serum protein analysis with proper consideration to such variables as drying of the paper strips, type of protein stain employed, time of staining and rinsing, and method of analyzing the completed and stained electrophoretic record. Considerable attention is given the method of analyzing stained records by photometric scanning, and the many possible sources of error are adequately pointed out. A description is given of the methods of studying nonprotein substances or protein-nonprotein complexes such