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August 1957

Chloramphenicol-Tetracycline Treatment of Salmonellosis in Children: Strip-Gradient and Replica Strip-Gradient Techniques as Guides to Therapy

Author Affiliations

Miami, Fla.
From the Department of Medical Research, National Children's Cardiac Hospital, the School of Medicine and the Department of Microbiology, University of Miami.

AMA Am J Dis Child. 1957;94(2):155-168. doi:10.1001/archpedi.1957.04030030049009

Chloramphenicol treatment, today the therapy of choice against typhoid fever and other Salmonella infections, is often inadequate in that relapses are common,1-3 the organisms persist in bile or stools for variable periods of time, carrier states may not be permanently eradicated, and the important complications of intestinal hemorrhage and perforation still may occur.4 The use of an additional antibiotic against Salmonella infections, so that the dose of each drug might be decreased and the efficacy increased, therefore appeared to us to be a logical approach to more adequate treatment. The choice of such an additional drug would depend upon the sensitivity of salmonellae to combinations of the available antibiotics.

To assist in selection of such an antibiotic combination, many methods are available. One is that of clinical trial. Another is that of in vitro testing; commonly, the serial tube dilution and plating method.5,6 This latter technique is