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June 1959

Protein Studies in Normal Newborn Infants: Paper Electrophoresis of Cerebrospinal Fluid and Serum

Author Affiliations

New York
Departments of Anatomy and Pediatrics, New York Medical College.

AMA Am J Dis Child. 1959;97(6):785-789. doi:10.1001/archpedi.1959.02070010787004

Serum protein fractions have been studied intensively in the normal newborn infant.1-9 However, there is only sparse information on the cerebrospinal fluid protein fractions in this age group.10-18 The concentration of the low protein content in small amounts of cerebrospinal fluid has presented a major problem. Earlier studies primarily used precipitation methods which could not separate the individual fractions. The use of dialysis methods for concentrating proteins, combined with paper electrophoresis, now affords adequate means for separation of the various components of cerebrospinal fluid proteins.

Paper electrophoretic studies of cerebrospinal fluid have been performed for the most part only in adults and older children,19-22 with few reports in normal newborn infants.17,18 The purpose of this study was to determine the distribution and relationship of proteins in the cerebrospinal fluid and serum of normal newborn infants utilizing chemical and paper electrophoretic methods.

Method  Thirty-five normal newborn infants