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December 1959

Preparation of P32-Labeled Candida Albicans

Author Affiliations

From the Department of Pediatrics, Maimonides Hospital.

AMA Am J Dis Child. 1959;98(6):765-767. doi:10.1001/archpedi.1959.02070020767012

Introduction  Determination of the route of infection and method of transmission of pathogenic organisms from one host to another has so far been a matter of inference rather than exact proof, since there is no way of distinguishing a given strain of a pathogen from other strains of the same species or type."Tagging" an infectious strain with radioactive material in order to render it distinguishable from others of the same species might offer a means of tracing such an organism through successive hosts.It was therefore attempted to prepare a "tagged" pathogen, and to determine the length of time through which radioactivity would remain detectable in vitro in successive subcultures from the original labeled strain. Candida albicans, the etiologic agent of thrush, was used for these experiments.Radioactive phosphorus (P32) was chosen as a "label," since it is a beta rather than a gamma emitter, with a maximum