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February 1961

Fluorescent Antibody Techniques: Methods for Identification of Bordetella Pertussis

Author Affiliations

Department of Epidemiology, School of Public Health, University of Michigan (Dr. Kendrick and Dr. Eveland); Michigan Department of Health Laboratories, Grand Rapids Branch, Grand Rapids (Dr. Eldering)

Am J Dis Child. 1961;101(2):149-154. doi:10.1001/archpedi.1961.04020030013003

The diagnosis of early, mild, or atypical whooping cough is dependent upon identification of the etiologic agent. Although procedures for the isolation of Bordetella pertussis1 have been available for many years and are of proven diagnostic aid, they have not gained the wide usage they seem to deserve. A recognized limitation is the time needed: 3, more often 4, and occasionally more, days are required for a positive report. The possibility of using fluorescent antibody methods for rapid identification of B. pertussis in nasopharyngeal specimens is suggested by the promising results reported in recent studies of a number of bacterial infections. For example, Group A streptococci,2,3 enteropathogenic Escherichia coli,4 Haemophilus influenzae, Type B,5 Neisseria gonorrhoeae,6 Vibrio comma,7 Leptospira,8 and certain other bacterial agents have been demonstrated by means of this technique in specimens taken directly from the patient. The work of de Repentigny

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