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February 1961

Immunofluorescence in Diagnostic Bacteriology: II. Identification of Group A Streptococci in Throat Smears

Author Affiliations

From The Child Research Center of Michigan and the Children's Hospital of Michigan.

Am J Dis Child. 1961;101(2):160-163. doi:10.1001/archpedi.1961.04020030024005

Recent investigations have shown the feasibility of applying the fluorescent antibody method to Lancefield grouping of streptococci.1-4 The present study was undertaken to test the application of this method to organisms found in direct smears made from throat swabs. The advantages of the use of direct smears over the use of cultures for the immediate detection of Group A streptococci are obvious; detection from direct smears permits prompt initiation of therapy for acute streptococcal pharyngitis or for epidemiologic surveys and control of rheumatic fever patients.

Materials and Methods  Throat smears were obtained from patients in the wards, emergency room, outpatient department, and rheumatic fever clinic of the Children's Hospital of Michigan, from families of children with known streptococcal infection, and from selected pediatric patients in the emergency room of Receiving Hospital. Reference cultures of Groups A and C β-hemolytic streptococci were obtained from the American Type Culture Collection.Two

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