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July 1961

Immunofluorescence in Diagnostic Bacteriology: III. The Identification of Enteropathogenic E. Coli Serotypes in Fecal Smears

Author Affiliations

From the Child Research Center of Michigan and the Children's Hospital of Michigan.

Am J Dis Child. 1961;102(1):82-90. doi:10.1001/archpedi.1961.02080010084013

For epidemiologic and clinical control of infantile diarrhea caused by enteropathogenic Escherichia coli (E.E.C.), rapid detection and identification of the etiologic serotype is essential.1,11 Conventional procedures for even presumptive identification of the organisms take from 24 to 48 hours at best. Moreover, since a specific selective culture medium for isolation of E.E.C. is not available,2 it is difficult to select E.E.C. colonies for serotyping from mixtures of enteric organisms existing in infant carriers, adult carriers, or treated infants. Fluorescent antibody techniques appeared to be ideally suited for an approach to the solution of these problems.

A previous report from this laboratory3 described the use of fluorescein-labeled antibodies for identifying E. coli 0127:B8 in fecal smears from an outbreak of diarrhea caused by that organism. Thomason et al.4 confirmed these results by utilizing polyvalent-labeled antisera representing 9 E.E.C. serotypes to identify E. coli 0127: B8

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