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March 1962

Homeothermic Cell Culture in Vitro

Author Affiliations

Charles Chany, M.D., Laboratoire des Virus, Hôpital St.-Vincent-de-Paul, Paris, France.; Centre de Récherches sur les Virus, Hôpital Saint-Vincent-de-Paul, and Departement des Virus de l'Institut Pasteur (Dr. Chany); Centre de Récherches sur les Leucemies, Hôpital Saint-Louis (Dr. Thomas).

Am J Dis Child. 1962;103(3):319-324. doi:10.1001/archpedi.1962.02080020331027

Introduction  If the central body temperature is characteristic for the species in homeothermic animals, one could assume that the same temperature will apply also in primary tissue culture. This very simple hypothesis suggested the study of 3 problems: (1) Is the body temperature of an animal the optimal temperature for cell multiplication in tissue culture? (2) What are the maximum and minimum temperatures in which the cells can multiply without visible cytological changes? (3) What is the effect of hyperthermia on the multiplication of latent viruses??Since some of the viral vaccines are produced in monkey kidney cells, this study was focused on the multiplication of monkey kidney cells at different temperatures and on the effect of hyperthermic cultures on latent monkey viruses. Some of this information has already been reported in a preliminary publication.1

Materials and Methods  Monkey kidney (Erythrocebus patas and cynocephalus) cells were trypsinized in the