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Article
August 1962

Identification of Group A Streptococci: Bacitracin Disc and Fluorescent Antibody Techniques Compared with the Lancefield Precipitin Method

Author Affiliations

DENVER
From the Departments of Pediatrics and Medicine, the University of Colorado School of Medicine, the Colorado State Department of Public Health, and the Denver General Hospital.

Am J Dis Child. 1962;104(2):157-160. doi:10.1001/archpedi.1962.02080030159008
Abstract

During the course of a project which had as its primary objective the evaluation of a streptococcal disease control program,1 an opportunity was presented to compare the efficacy of (a) the bacitracin disc method and (b) the fluorescent antibody method of identifying Group A streptococci with the Lancefield precipitin technique. The results which were obtained and are here presented are confirmatory of previous work2,3 and show that both the bacitracin disc and the fluorescent antibody techniques are highly reliable. Each, therefore, represents a useful method for the identification of Group A streptococci in clinical laboratories.

Methods  A total of 776 strains of streptococci was subjected to study; 669 (90%) were isolated from patients who were seen on the clinical services of the Denver General Hospital, while 69 strains (10%) were obtained from household contacts of patients included in the study. Of the streptococcal isolations, 76% were made from

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