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May 1963

Hemophilus Influenzae in Respiratory Infections: I. Typing by Immunofluorescent Techniques

Author Affiliations

Sarah H. Wood Sell, M.D., Department of Pediatrics, Vanderbilt University School of Medicine, Nashville 5, Tenn.; Assistant Professor of Pediatrics (Dr. Sell); Assistant Professor of Pathology (Dr. Cheatham).; From the Departments of Pediatrics and Pathology, Vanderbilt University School of Medicine.

Am J Dis Child. 1963;105(5):466-469. doi:10.1001/archpedi.1963.02080040468007

The role played by Hemophilus influenzae in respiratory infections is poorly understood. In contrast to the single type usually identified in cerebrospinal fluid of acute meningitis, the strains isolated from the respiratory tract during acute infection constitute a heterogeneous group, few of which are typable by antisera prepared against the known types (a-f of Pittman's classification).1 Both the typability 1 and the pathogenicity 2 appear to be related to the presence of specific capsular antigens.

The success, in recent years, of fluorescent antibody techniques in diagnostic bacteriology and serology 3 suggested the possible application of these new methods to the evaluation of the role of H. influenzae in respiratory infections. Page, Caldroney, and Stulberg4 reported demonstration, by immunofluorescence, of Type b H. influenzae in the spinal fluid from children with meningitis. The technique has also been used to study the bacteriologically related pertussis organism.5,6 Since antibodies can

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