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October 1966

Bilirubin in the Liver and Kidney in Jaundiced Rats

Author Affiliations

From the Department of Pediatrics of the Johns Hopkins University School of Medicine and the Harriet Lane Service of the Johns Hopkins Children's Medical and Surgical Center.

Am J Dis Child. 1966;112(4):351-358. doi:10.1001/archpedi.1966.02090130125012

IN A PREVIOUS balance of bilirubin from this laboratory, retention of bilirubin occurred in extravascular tissue spaces.1 When 8 mg of bilirubin/100 gm of mature rat was infused during a 60-minute interval, the animals excreted 40%. Of the retained bilirubin, 25% was accounted for by direct analysis in the plasma and 7% in adipose tissue. The remaining 68% of the retained bilirubin was presumed to be in extravascular tissue spaces which were non-adipose. This "residual" bilirubin was derived by arithmetic difference from the balance rather than from direct analysis. A more direct measurement of the residual bilirubin and particularly its intracellular distribution was desired.

One obvious location for significant quantities of infused bilirubin is in the liver. A number of investigations have demonstrated the rapidity with which the liver can remove bilirubin from the circulating plasma and excrete it in bile as its conjugated derivatives,2-4 Schmid and co-workers

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