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July 1969

Purification and Characterization of Rubella Virus Hemagglutinins

Author Affiliations

From the Department of Virology, Karolinska Institutet, Medical School, Stockholm.

Am J Dis Child. 1969;118(1):94-100. doi:10.1001/archpedi.1969.02100040096016

THE hemagglutinating activity of untreated rubella virus material is mainly carried by complete virus particles.1-3 These have a size of 40 to 60 mμ2,4 and a sedimentation constant of 240 S.1,5

A subunit hemagglutinin, obtained after treatment with polysorbate 80 (Tween 80) and ether, has been characterized by sedimentation analysis.2,5 The average sedimentation constant of this hemagglutinin was 100 S. The particles varied in size with an average diameter of 25 mμ as estimated from filtration experiments.2

The purpose of the present study was to find alternative disintegration methods for rubella virus and to further characterize the subunit hemagglutinins.

Materials and Methods  Virus Strain.—A strain of rubella virus isolated by Dr. Harvey Liebhaber, Yale University, New Haven, Conn, was used.Tissue Cultures.—VERO cells were propagated in roller bottles with a growth medium consisting of Eagle's basal medium supplied with 10% calf serum.Infection