To evaluate an optical immunoassay rapid antigen test to detect group A β-hemolytic streptococci directly from throat swab specimens.
Criterion standard with "blinded" comparison. Double-swab pharyngeal samples were obtained; one swab was cultured and the other was used for the rapid antigen test.
Microbiology laboratory in a primary care center at a university teaching hospital.
Two hundred sixty-two outpatients with pharyngitis.
Main Outcome Measure:
The results of the optical immunoassay rapid antigen test were compared with results of standard aerobic culture.
Sixty-two (24%) of 262 samples were culture-positive; 48 of these 62 were positive by the optical immunoassay rapid test (sensitivity, 77%). Of the 200 culture-negative samples, seven (4%) were positive by the rapid test (specificity, 96%).
The optical immunoassay performed well, but like other rapid tests, is not sensitive enough to replace standard culture for detection of group A β-hemolytic streptococci.(Arch Pediatr Adolesc Med. 1996;150:245-248)
Schlager TA, Hayden GA, Woods WA, Dudley SM, Hendley JO. Optical Immunoassay for Rapid Detection of Group A β-Hemolytic Streptococci: Should Culture Be Replaced? Arch Pediatr Adolesc Med. 1996;150(3):245–248. doi:10.1001/archpedi.1996.02170280015002
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