Salience Network–Based Classification and Prediction of Symptom Severity in Children With Autism

Importance  Autism spectrum disorder (ASD) affects 1 in 88 children and is characterized by a complex phenotype, including social, communicative, and sensorimotor deficits. Autism spectrum disorder has been linked with atypical connectivity across multiple brain systems, yet the nature of these differences in young children with the disorder is not well understood.

Objectives  To examine connectivity of large-scale brain networks and determine whether specific networks can distinguish children with ASD from typically developing (TD) children and predict symptom severity in children with ASD.

Design, Setting, and Participants  Case-control study performed at Stanford University School of Medicine of 20 children 7 to 12 years old with ASD and 20 age-, sex-, and IQ-matched TD children.

Main Outcomes and Measures  Between-group differences in intrinsic functional connectivity of large-scale brain networks, performance of a classifier built to discriminate children with ASD from TD children based on specific brain networks, and correlations between brain networks and core symptoms of ASD.

Results  We observed stronger functional connectivity within several large-scale brain networks in children with ASD compared with TD children. This hyperconnectivity in ASD encompassed salience, default mode, frontotemporal, motor, and visual networks. This hyperconnectivity result was replicated in an independent cohort obtained from publicly available databases. Using maps of each individual’s salience network, children with ASD could be discriminated from TD children with a classification accuracy of 78%, with 75% sensitivity and 80% specificity. The salience network showed the highest classification accuracy among all networks examined, and the blood oxygen–level dependent signal in this network predicted restricted and repetitive behavior scores. The classifier discriminated ASD from TD in the independent sample with 83% accuracy, 67% sensitivity, and 100% specificity.

Conclusions and Relevance  Salience network hyperconnectivity may be a distinguishing feature in children with ASD. Quantification of brain network connectivity is a step toward developing biomarkers for objectively identifying children with ASD.

Autism spectrum disorder (ASD), a neurodevelopmental disorder that affects language, social communication, motor behaviors, and sensory systems, affects nearly 1 in 88 children.¹ Increasingly, ASD is understood to be associated with atypical development of multiple interconnected brain systems rather than isolated brain regions.² Although converging lines of research have demonstrated altered brain connectivity in individuals with autism,^3,4 inconsistency with respect to analytic techniques remains an issue.⁵ One prevailing theory posits that autism is caused by underfunctioning integrative circuitry, resulting in information integration deficits at the neural and cognitive levels.⁶ The underconnectivity theory is based largely on analysis of task-related changes in interregional connectivity during tasks that involve language,^6,7 working memory,⁸ mental imagery,⁹ executive functions,¹⁰ cognitive control,^11,12 and social cognition.^13,14 However, several reports of brain hyperconnectivity in ASD also exist in the domains of visuomotor processing,^15,16 visual search,¹⁷ emotion processing,¹⁸ memory,¹⁹ and language.²⁰ More recently, evidence for both underconnectivity and hyperconnectivity in ASD has come from intrinsic connectivity studies^21-24 using resting-state functional magnetic resonance imaging (fMRI). These contradictory findings leave open the question of whether and to what extent intrinsic functional connectivity of the brain is altered in autism and suggest that both hypoconnectivity and hyperconnectivity may underlie the complex phenotype of the disorder.²⁵ At the cognitive and behavioral levels, ASD is characterized by difficulties with integration across domains, rigidity, repetitive behaviors, and hypersensitivity. How such behaviors may arise from hypoconnected or hyperconnected brain systems remains unknown. Although relatively few studies^21,26 have directly linked aberrant intrinsic brain connectivity in ASD to specific symptoms, there is a growing literature that demonstrates that individual differences in intrinsic functional connectivity are systematically related to measures of empathy,²⁷ IQ,²⁸ and behavioral variability²⁹ in neurotypical adults. To date, no studies have systematically explored whether connectivity is diminished or enhanced within specific large-scale functional brain systems^30,31 in children with ASD.

Autism is a disorder with early life onset and variable developmental trajectory³²; therefore, studies of young children are especially critical for developing accurate models of the underlying neurobiology of the disorder. Indeed, at earlier developmental time points, a quite different picture of brain connectivity in autism is observed. One of the earliest signs of autism is enlarged head circumference or macrocephaly.³³ Infants and young children with ASD show signs of early brain overgrowth.³⁴ Although the relationship between neuron number and brain size is complex, postmortem studies³⁵ of children with ASD indicate that they have an overabundance or excess numbers of neurons in the prefrontal cortex. Some of these differences are diminished with development, such that older children,³⁶ adolescents, and adults with autism do not differ from neurotypical individuals on measures of brain size.³⁷ This finding suggests a different developmental trajectory in ASD, underscoring the point that group differences observed in adults cannot necessarily be extrapolated to understand the neurobiology of childhood autism. A study³⁸ of functional connectivity during performance of a response inhibition task found no differences between children with ASD and typically developing (TD) children. The only published study²⁴ examining intrinsic functional connectivity of the brain in children with ASD found hyperconnectivity between subcortical regions and heteromodal association cortex.

Accurate characterization of brain connectivity in children with autism is a first step toward developing brain-based biomarkers for the disorder. To date, only one study³⁹ has used intrinsic functional connectivity to attempt to classify individuals with autism. For participants younger than 20 years, whole-brain functional connectivity measures could discriminate between individuals with ASD and control participants, but classification accuracies were much lower for older participants. This finding further highlights the need for investigations targeting younger individuals.

No previous studies have examined the complete range of large-scale brain networks^30,31 in children with autism. We examine whole brain intrinsic functional connectivity in children with ASD using independent component analysis (ICA), a data-driven, unbiased approach for uncovering coherent and highly reproducible large-scale brain networks.⁴⁰ We sought to determine whether children with ASD showed patterns of functional connectivity more consistent with hyperconnectivity or hypoconnectivity accounts and to test whether measures of functional connectivity could be used to discriminate children with ASD from TD children. On the basis of a recent systems-level theoretical model of social and cognitive dysfunction in ASD,⁴¹ we hypothesized that the salience network, composed of the anterior insular cortex and anterior cingulate cortex (ACC), would show aberrant patterns of brain connectivity and provide the greatest information regarding group membership. Regions within the salience network are implicated in multiple functions, ranging from attention to interoception and subjective awareness.^42,43 The salience network integrates external sensory stimuli with internal states,⁴⁴ and we have previously reported that it is critical for orchestrating brain network dynamics.^45-47 Network analyses indicate that the anterior insula acts as a hub, mediating interactions between large-scale networks involved in externally and internally oriented cognitive processing,⁴⁶ and a meta-analysis of neuroimaging studies of social cognition identifies the anterior insula as a consistent locus of hypoactivity in autism.⁴⁸ Synthesizing previous work, we propose that a more general role of the salience network is attention allocation to stimuli that is salient to the individual⁴⁹ and hypothesize that atypical development of the salience network may contribute to diminished interest in social interaction, a hallmark of ASD.⁴¹ The current study is the first to examine the salience network in this context.

Participants were recruited from the San Francisco Bay Area through advertisements. Children with ASD were also recruited from the Stanford Autism Clinic and the Lucille Packard Children’s Hospital. Children were evaluated using the Autism Diagnostic Interview–Revised (ADI-R)⁵⁰ and the Autism Diagnostic Observation Schedule (ADOS)⁵¹ administered by a trained research assessor, and diagnoses were confirmed by a clinical psychologist (J.P.). All participants had scores in the autism range on the ADI-R with the exception of one child whose scores were slightly below the cutoffs for communication (2 points) and repetitive behavior (1 point) and one child whose score was 1 point below the cutoff for repetitive behavior. In both cases, these children fell within the ASD range on the ADOS and were therefore considered to fall within the ASD range using the combination of scores on these measures.⁵² On the ADOS, 9 of the participants had scores in the ASD range and 10 of the participants had scores in the autism range. The ADOS scores were not available for one of the participants, whose ADI-R score fell well within the autism range. All participants were verbal and high functioning, as evidenced by their relatively high IQ scores. All participants underwent standardized neuropsychological assessments, including the Wechsler Abbreviated Scale of Intelligence (eMaterials in the Supplement).

Neuroimaging data were collected from 33 children with ASD. Data from participants who exhibited greater than 5 mm of motion in any direction during fMRI were not considered for further analysis. The final sample included 20 children with ASD (16 boys and 4 girls; mean [SD] age, 9.96 [1.59] years; full-scale mean [SD] IQ, 112.6 [17.8]). Using an algorithm developed to select control participants from a preexisting data set (eMaterials), 20 children matched on age, sex, and IQ were selected (16 boys and 4 girls; mean [SD] age, 9.95 [1.60]; full-scale mean [SD] IQ, 112.1 [15.4]; Table 1). Participants gave written informed assent, and parents or guardians gave written informed consent. Additional information regarding current medications and comorbid psychopathologic conditions are provided in the eMaterials. The study was approved by the Stanford University Institutional Review Board.

Before resting-state fMRI, children viewed the following instructions: “Relax. Please keep your eyes closed but do not go to sleep.” They were also instructed to try not to move for the duration of the 6-minute scan. Functional images were acquired on a 3T GE Signa scanner (General Electric) using a custom-built head coil. Head movement was minimized during scanning by small cushions. A total of 29 axial sections (4.0-mm thickness, 0.5-mm skip) parallel to the anterior commissure—posterior commissure line and covering the whole brain were imaged using a T2-weighted gradient echo spiral in-out pulse sequence⁵³ with the following parameters: repetition time, 2000 milliseconds; echo time, 30 milliseconds; flip angle,  80°; and 1 interleave, resulting in 180 volumes. The field of view was 20 cm, and the matrix size was 64 × 64, providing an in-plane spatial resolution of 3.125 mm. To reduce blurring and signal loss arising from field inhomogeneities, an automated high-order shimming method based on spiral acquisitions was used before acquiring functional scans.

For each participant, a high-resolution T1-weighted spoiled grass gradient recalled inversion recovery 3-dimensional MRI sequence was acquired (inversion time, 300 milliseconds; repetition time, 8.4 milliseconds; echo time, 1.8 milliseconds; flip angle,  15°; 22-cm field of view; 132 sections in coronal plane; 256 × 192 matrix; 2 excitations, acquired resolution, 1.5 × 0.9 × 1.1 mm).

The fMRI data were preprocessed using SPM statistical software, version 8 (http://www.fil.ion.ucl.ac.uk/spm). Details of preprocessing steps, results from analyses implementing the data “scrubbing” procedure for removing motion artifacts,⁵⁴ and additional analyses are presented in the eMaterials, eFigure 1, eFigure 2, and eFigure 3 in the Supplement.

We used the dual regression approach implemented in FSL (www.fmrib.ox.ac.uk/analysis/dualreg/).⁵⁵ This method has recently emerged as the preferred option for comparing large-scale brain networks between groups.^56-59 First, preprocessed data from both groups (TD and ASD) were concatenated and entered into a group ICA to identify large-scale patterns of functional connectivity in the population. Data were decomposed into 25 independent components using MELODIC (http://fsl.fmrib.ox.ax.uk/fsl/fslwiki/MELODIC). Second, 10 components corresponding to previously described functional networks were selected (Figure 1). The salience network, with key nodes in the dorsal ACC and frontoinsular cortices, is thought to be a nexus that unites conflict monitoring, interoceptive-autonomic, and reward processing centers.⁴⁴ The right central executive network is anchored in the dorsolateral prefrontal cortex and posterior parietal cortex and is involved in the maintenance and manipulation of information and decision making in the context of goal-directed behavior.⁴⁶ We found 3 components resembling the default mode network (DMN), with key nodes in medial prefrontal cortex and posterior cingulate cortex. These components have previously been described as the posterior DMN, the ventral DMN, and the anterior DMN⁵⁷ in studies of older populations. One study⁶⁰ comparing children and adults demonstrated separation of the DMN into 2 components. DMN function encompasses a range of self-relevant and social cognitive processes.⁵⁸ The dorsal attention network is a bilateral system with nodes in the intraparietal sulcus and frontal eye fields and is involved in top-down orienting of attention.⁶¹ The motor network comprises regions of precentral gyrus and supplementary motor area.⁶² Primary and association visual networks were observed,⁵⁸ spanning medial and lateral surfaces of the occipital lobe, respectively. We also observed a frontotemporal network⁵⁸ with nodes in bilateral superior temporal gyri and inferior frontal gyri. The remaining components corresponded to obvious noise and artifacts (eg, white matter, ventricles, eyes, and motion) and were not further analyzed.

Third, the dual-regression algorithm was applied to identify participant-specific time courses and spatial maps (eMaterials in the Supplement). Group difference maps from this statistical testing were thresholded using threshold-free cluster enhancement at the P < .05 level.

The 10 components identified from each participant (described above) served as features to be input into classification analyses. We used a logistic regression classifier (implemented in the Matlab package GLMnet; http://www-stat.stanford.edu/~tibs/glmnet-matlab) to classify children with ASD and TD children (eMaterials in the Supplement). We report the cross-validation accuracy, sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) for each of the 10 networks. Permutation tests were conducted to arrive at P values associated with classification accuracies for each network. Sensitivity measures the proportion of positive results that are correctly identified as such, in this case, the percentage of children with ASD who are correctly identified as having ASD. Specificity measures the proportion of negative results that are correctly identified, or the percentage of TD children who are correctly identified as TD.

To investigate brain-behavior associations, we used a sparse regression algorithm (eMaterials in the Supplement). The sparse regression algorithm identifies voxels that predict symptom severity by modeling the relationship between the dependent variable (score on ADOS or ADI subscale) and the independent variables (voxels within a network). GLMnet (http://www-stat.stanford.edu/~tibs/glmnet-matlab/), a state-of-the-art sparse regression algorithm,⁶³ was also used in this analysis. R² was used to measure the performance of the regression algorithm in predicting symptom severity.

From the initial group ICA (n = 40), 10 of the 25 components were visually identified as corresponding to previously described functional networks^40,64 (Figure 1, eFigure 1, and eFigure 2 in the Supplement). Significant group differences were found in 6 of the 10 functional networks examined: the salience, posterior DMN, frontotemporal, motor, and visual networks. For each of these networks, there was greater connectivity in children with ASD compared with TD children, or hyperconnectivity in ASD (Figure 2). Within the salience network, ASD greater than TD functional connectivity was observed in the ACC, superior frontal gyrus, thalamus, and bilateral insular cortices. Within the posterior DMN, ASD greater than TD functional connectivity was observed in the precuneus, posterior cingulate, and left angular gyrus. Within the frontotemporal network, there was ASD greater than TD functional connectivity in bilateral superior and middle temporal gyri. Within the motor network, there was ASD greater than TD functional connectivity in bilateral precentral and postcentral gyri, left posterior insula, and thalamus. Within the 2 visual networks, there was ASD greater than TD functional connectivity in the left lateral occipital cortex, intracalcarine cortex, and occipital pole. All group differences were observed within component boundaries, and none of the networks examined had differences in any brain areas when we examined the opposite contrast (TD greater than ASD functional connectivity). This network hyperconnectivity result was replicated in an independent data set of 15 children with ASD and 15 TD children obtained from public databases (http://ndar.nih.gov/ and http://fcon_1000.projects.nitrc.org/indi/adhd200/; eMaterials, eFigure 4, and eFigure 5 in the Supplement).

Maps corresponding to each of the 10 networks for each participant were individually evaluated for their ability to discriminate children with ASD from TD children using a classification algorithm based on a logistic regression framework⁶³ (Figure 3A). Classification accuracies, sensitivities, specificities, PPV, and NPV for each network are presented in Table 2. The salience network produced the highest classification accuracy of all networks, with 78% accuracy (P = .02), 75% sensitivity, and 80% specificity (PPV, 79%; NPV, 76%) (Figure 3B). Systematic variation of mask thresholds verified that the salience network produced the highest classification accuracy of all networks at all reasonable threshold values. Applying the salience network–based classifier to data from an independent cohort, 83% accuracy (P = .02), 67% sensitivity, and 100% specificity (PPV, 100%; NPV, 75%) were observed (eMaterials in the Supplement).

To examine the associations between ASD symptom severity and brain networks, we used a multivariate sparse regression analysis.⁶³ On the basis of our a priori hypotheses⁴¹ and the finding that the salience network exhibited both strong group differences and the highest classification accuracy for discriminating ASD from TD, we focused on exploring relationships with this specific network. We found that the salience network was related to restricted and repetitive behaviors as measured by the ADI-R in that voxels within the network predicted severity of symptoms (R² = 0.36, P = .007, Bonferroni corrected). No significant relationships were observed between the salience network and scores on the other subscales of the ADOS or ADI.

Autism is a complex neurodevelopmental disorder that affects multiple cognitive domains and brain systems. Questions regarding the nature (hypoconnectivity or hyperconnectivity), extent (widespread or focal), and developmental timeframe (childhood or adulthood) of aberrant brain connectivity in autism are hotly debated. We address a critical gap in the literature by examining whole-brain intrinsic functional connectivity in children with ASD and identifying the specific brain network that most successfully discriminates children with ASD from TD peers. We found that childhood autism is characterized by hyperconnectivity of major large-scale brain networks and that the salience network may be a distinguishing feature in children with ASD.

We used ICA, a data-driven approach for assessing group differences in functional brain networks, to analyze fMRI data from children with ASD and TD children. Across 6 of the 10 brain networks identified in the combined sample, children with ASD exhibited greater functional connectivity than TD children. The salience network, DMN, frontotemporal network, motor network, and visual network all demonstrated greater functional connectivity in children with ASD compared with TD children. The opposite pattern of results (TD greater than ASD functional connectivity) was not observed in any brain region for any of the networks examined. This pattern of results was replicated in an independent cohort of children with ASD and TD children.

The underconnectivity theory of autism posits that the disorder results from reduced functional connectivity between frontal and posterior cortical regions.^6,65,66 It is not yet clear how connectivity measures can be affected by methodologic choices.⁵ In addition, few studies have addressed the question of how the brain is functionally organized in childhood autism, at times more proximal to the onset of the disorder.³⁷ We report hyperconnectivity of several major large-scale brain networks in children with autism. We found that in the salience network, children with ASD showed hyperconnectivity, specifically in the ACC and bilateral insular cortices. This network is involved in interoceptive and affective processes and the identification of relevant internal and extrapersonal stimuli to guide behavior.^44,49 We previously hypothesized that atypical functional connectivity of the insula may be part of the neuropathology of ASD.⁴¹ It has since been demonstrated that adolescents with ASD show decreased regional homogeneity (a measure of local synchronization of fMRI signal) in the right insula.⁶⁷ Investigations using region of interest–based approaches have shown decreased resting-state functional connectivity between the insula and ACC in adolescents with autistic disorder.⁶⁸ The insula-ACC pathway is of particular interest in ASD because it has previously been shown that this link undergoes significant functional and structural changes throughout typical development,⁴⁶ and functional connectivity along this pathway is related to measures of social responsiveness in typical adults.⁶⁹ We have proposed that the salience network may serve a general purpose to detect salient endogenous or exogenous events and initiate and mobilize resources for appropriate behavioral responses.⁴⁹ Dysfunction of this network could in principle lead to some of the core features of ASD, including reduced attention to social stimuli.⁴¹

We also found evidence for hyperconnectivity of the posterior DMN in children with ASD. The DMN is involved in many of the processes that are compromised in individuals with autism, including social and interpersonal cognition.^70,71 Our current findings contrast with previously published studies^21-23,26 of DMN hypoconnectivity in adults and adolescents with ASD, underscoring the critical need for examining brain connectivity in younger children, where a different picture is beginning to emerge. We observed hyperconnectivity of the posterior DMN, which contains regions thought to be involved in episodic memory and self-related processing.⁷² Aberrant connectivity of the DMN in childhood autism may hinder the development of processes, such as reciprocal social interaction, that require flexible and adaptive responses and integration of current goals with past experience.

Brain hyperconnectivity in ASD was also observed within frontotemporal, motor, and visual networks. The superior temporal sulcus, involved in various aspects of biological motion processing, is a region that has been extensively implicated in ASD for its role in action understanding and social cognition⁷³ and speech perception.⁷⁴ Dyspraxia and other impairments of motor control have also been reported,⁷⁵ as well as sensory processing abnormalities.^76,77 We observed hyperconnectivity across brain systems supporting several cognitive functions known to be impaired in the complex autistic phenotype.

Taken together, these results suggest that brain network hyperconnectivity is a critical component of the underlying neurobiology of childhood ASD. Our work suggests that the large body of literature describing hypoconnectivity between brain regions in adult ASD may not be representative and obscures the full, complex developmental sequelae of the disorder. The functional implications of hyperconnectivity within brain networks are far-reaching. If enhanced within-network connectivity is characteristic of autism, this may limit dynamic interactions among networks, which are necessary for successful navigation of complex real-world scenarios. Thus, we speculate that network isolation may account for some of the core symptoms of ASD, namely, difficulty with adapting to change, restrictive and repetitive behavior,⁷⁸ and increased sensitivity to visual, auditory, and tactile stimuli.⁷⁹

The current results provide hints of an important developmental discontinuity in ASD, the underlying mechanisms of which are currently unknown. Although this idea is speculative, there might be a critical period in development, possibly during puberty, that affects brain organization in ASD differently than in TD children. It has been shown that ovarian hormones enhance both corticocortical and subcorticocortical functional connectivity, whereas androgens decrease subcorticocortical functional connectivity but increase functional connectivity among subcortical brain areas.⁸⁰ These developmental factors have not yet received adequate attention. Whereas we find hyperconnectivity in 7- to 12-year-old children with ASD, most of the studies in the intrinsic connectivity^16-18,26,81 and task-related connectivity literature^6-8,10,12 find hypoconnectivity. Because almost all of the existing literature on functional brain connectivity in ASD reports data from adolescents and adults, rather than children with the disorder, it is possible that this critical developmental discontinuity has gone unrecognized.

The current findings suggest that at earlier ages closer to onset of the disorder, the brain in ASD is largely hyperconnected. In addition, this hyperconnectivity can be quantified in ways that may eventually be used to discriminate children with ASD from TD children. Only one published study³⁹ has evaluated resting-state functional connectivity–based biomarkers. Pairwise functional connectivity measures derived from 7266 regions of interest across gray matter could be used to distinguish adolescents and adults with autism from typically developing individuals. Our results suggest that specific, anatomically and functionally well-characterized brain networks⁸² contain more information regarding group membership than others. Previous work used measures of brain structure, such as cortical thickness,⁸³ gray matter volume,⁸⁴ or white matter integrity,⁸⁵ to attempt to discriminate individuals with ASD from TD individuals with varying degrees of success. Because these studies have all examined adolescents or adults, their utility in identifying brain-based biomarkers in younger children remains unclear. We demonstrate that large-scale brain network connectivity can predict clinical category in children.

Among the 10 networks examined, we found that the salience network had the greatest classification accuracy at 78%, with 75% sensitivity and 80% specificity. Furthermore, the classifier trained on the salience network in the current data set could generalize to discriminate children with ASD from TD children in an independent data set with 83% accuracy, 67% sensitivity, and 100% specificity. The salience network functions to identify relevant internal and extrapersonal stimuli to guide behavior.⁴⁴ Dysfunction of this system may be part of the underlying neurobiology of autism.⁴¹ Increasingly, the anterior insula and ACC, the 2 main nodes of the salience network, are linked to behaviors affected in autism ranging from social perception^86,87 to cognitive control.⁸⁸ The other networks examined produced classification accuracies of 58% to 73%. Thus, although several networks exhibited hyperconnectivity in ASD, some were much more informative than others for providing information that may eventually be used in the development of brain-based biomarkers. The salience network was further linked to ASD symptoms. Information contained within the salience network could predict scores on measures of restricted and repetitive behaviors. The relationship between the salience network and restricted and repetitive behaviors might be due to the more general role of this network in attention allocation to stimuli that is salient to the individual.⁴⁹ Regions that belong to this network are also involved in the maintenance of task sets during goal-directed behavior,⁸⁹ an excess of which might contribute to restricted and repetitive behaviors. Thus, our findings link for the first time, to our knowledge, a novel description of the neurobiology of childhood ASD with the identification of a potential brain-based biomarker that is related to the core symptoms of the disorder.

It is encouraging that the only other existing study of classification in autism using resting-state fMRI demonstrated that the brain regions most informative for classification included bilateral anterior insular cortices.³⁹ This result is in line with our finding that the salience network discriminated groups with the highest classification accuracy of all networks examined. The authors of the previous study used a different approach for assessing group differences in functional connectivity, namely, pairwise correlations among regions of interest covering cortical gray matter. Despite considerable methodologic differences and differences in age and sample characteristics, these highly discriminating brain regions in ASD were identified across both studies. The various available methods for processing resting-state fMRI data each have their own strengths and weaknesses, and as a whole these methods can provide complementary information about brain functional organization in neurodevelopmental disorders.⁹⁰

Although the results of brain hyperconnectivity in ASD replicate across 2 independent data sets, a limitation of the current study is the sample size and the relatively high IQ of the children investigated. Future work is necessary to extend these findings to larger sample sizes that include children on both ends of the autism spectrum. The current samples may not be representative of the diagnostic category as a whole in a disorder as heterogeneous as ASD. In addition, some of the older children in the current study may be old enough to be considered adolescents, although data on pubertal stage are not available to verify this. An additional limitation is the fact that it is not possible to know whether children with ASD interpreted the instructions during the resting-state scan differently from TD children. In addition, future studies will need to control for medication effects and effects of comorbidities. Finally, the inclusion of data from children with other developmental disorders will be essential for determining to what extent the classifier developed here can discriminate ASD from other disorders. The current study is a first step toward uncovering distinguishing features in the brains of children with ASD.

In conclusion, characterizing the nature of aberrant brain connectivity in ASD is necessary for understanding its complex behavioral and cognitive phenotype. We demonstrate brain functional hyperconnectivity in childhood ASD and report that this may be a signature of the disorder. We identify the salience network as a candidate biomarker in ASD and provide evidence that atypical connectivity of this network is related to deficits characteristic of the disorder. Future work is necessary for extending this finding to even younger children, with the ultimate goal of developing brain-based biomarkers that may be used to aid diagnosis and guide targeted early intervention.

Submitted for Publication: June 21, 2012; final revision received October 15, 2012; accepted November 20, 2012.

Corresponding Authors: Lucina Q. Uddin, PhD, or Vinod Menon, PhD, Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, 401 Quarry Rd, Stanford, CA 94305 (lucina@stanford.edu or menon@stanford.edu).

Published Online: June 26, 2013. doi:10.1001/jamapsychiatry.2013.104.

Author Contributions:Study concept and design: Uddin, Supekar, Menon.

Acquisition of data: Uddin, Lynch, Khouzam, Phillips.

Analysis and interpretation of data: Uddin, Supekar, Lynch, Feinstein, Ryali, Menon.

Drafting of the manuscript: Uddin, Supekar, Lynch, Khouzam, Menon.

Critical revision of the manuscript for important intellectual content: Uddin, Phillips, Feinstein, Ryali, Menon.

Statistical analysis: Uddin, Supekar, Ryali.

Obtained funding: Uddin, Menon.

Administrative, technical, and material support: Lynch, Khouzam, Phillips, Menon.

Study supervision: Uddin, Phillips, Menon.

Conflict of Interest Disclosures: None reported.

Funding/Support: This work was supported by National Institute of Mental Health Career Development Award K01MH092288 (Dr Uddin) and grants from the Singer Foundation, the Stanford Institute for Neuro-Innovation & Translational Neurosciences, and grants DC011095 and MH084164 from the National Institutes of Health (Dr Menon).

Role of the Sponsors: The funding organizations played no role in design and conduct of the study, collection, management, analysis, and interpretation of the data, and preparation, review, or approval of the manuscript.

Previous Presentation: Portions of these data were presented at the Annual Meeting of the Organization for Human Brain Mapping; June 14, 2012; Beijing, China.

Additional Contributions: Tianwen Chen, PhD, provided assistance with data processing and Maria Barth, BA, Christina Young, BA, Caitlin Tenison, BA, and Sangeetha Santhanam, BA, provided assistance with data collection. Data for the replication analyses were obtained from public databases (http://ndar.nih.gov/ and http://fcon_1000.projects.nitrc.org/indi/adhd200/) and were contributed by F. Xavier Castellanos, MD, and Michael P. Milham, MD, PhD.