The thickness of an edge reflects the magnitude of the correlation (the thickest edge representing a value of 0.61, which is the correlation between Positive and Negative Syndrome Scale [PANSS] positive [Pos] and PANSS disorganization [Dis]). Green lines represent positive correlations, whereas red lines represent negative correlations. Nodes with the same color belong to the same domain. Att indicates attention; Avl, Brief Negative Symptom Scale (BNSS) avolition; Dep, Calgary Depression Scale for Schizophrenia total score for depression; Dis, PANSS disorganization; ELS, everyday life skills; EnS, service engagement; ExD, BNSS expressive deficit domain; FC, functional capacity; FCo, family cohesion; FEI, Facial Emotion Identification Test; Inc, incentives; Int, interpersonal relationships; MSC, Measurement and Treatment Research to Improve Cognition in Schizophrenia Consensus Cognitive Battery Mayer-Salovey-Caruso Emotional Intelligence Test managing emotion section; PFu, perception of future; Pos, PANSS positive factor; PrS, problem solving; PSe, perception of self; PSp, processing speed; SCo, social competence; SLe, visuospatial learning; Stg, stigma; Ta1, The Awareness of Social Inference Test (TASIT) section 1 (see footnote a in Table 1 for a description of the sections); Ta2, TASIT section 2; Ta3, TASIT section 3; VLe, verbal learning; WMe, working memory; and Wrk, Specific Level of Functioning Scale work skills.
A, Betweenness indicates the number of times a node lies on the shortest path length between any 2 other nodes. B, Closeness indicates the average distance of a node from all other nodes in the network. C, Strength quantifies the extent to which a certain node influences other nodes in the network. For each index, higher values reflect greater centrality in the network. Att indicates attention; Avl, Brief Negative Symptom Scale (BNSS) avolition; Dep, Calgary Depression Scale for Schizophrenia total score for depression; Dis, Positive and Negative Syndrome Scale (PANSS) disorganization; ELS, everyday life skills; EnS, service engagement; ExD, BNSS expressive deficit domain; FC, functional capacity; FCo, family cohesion; FEI, Facial Emotion Identification Test; Inc, incentives; Int, interpersonal relationships; MSC, Measurement and Treatment Research to Improve Cognition in Schizophrenia Consensus Cognitive Battery Mayer-Salovey-Caruso Emotional Intelligence Test managing emotion section; PFu, perception of future; Pos, PANSS positive factor; PrS, problem solving; PSe, perception of self; PSp, processing speed; SCo, social competence; SLe, visuospatial learning; Stg, stigma; Ta1, The Awareness of Social Inference Test (TASIT) section 1 (see footnote a in Table 1 for a description of the sections); Ta2, TASIT section 2; Ta3, TASIT section 3; VLe, verbal learning; WMe, working memory; and Wrk, Specific Level of Functioning Scale work skills.
eAppendix 1. Methods
eAppendix 2. Analysis of Network Robustness and Stability
eTable. Zero-Order Correlation Matrix of the Variables Selected for the Network Analysis
eFigure 1. Example of a Network with 5 Nodes and 6 Edges
eFigure 2. Node Strength Centrality Estimates of the 27 Nodes Obtained by the Bootstrap Sampling Procedure
eFigure 3. Results of Case-Dropping Subset Bootstrap Procedure to Assess Stability of Network Centrality Indices
eFigure 4. Bootstrapped 95% Confidence Intervals of Edge Weights for the Network of 27 Variables
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Galderisi S, Rucci P, Kirkpatrick B, Mucci A, Gibertoni D, Rocca P, Rossi A, Bertolino A, Strauss GP, Aguglia E, Bellomo A, Murri MB, Bucci P, Carpiniello B, Comparelli A, Cuomo A, De Berardis D, Dell’Osso L, Di Fabio F, Gelao B, Marchesi C, Monteleone P, Montemagni C, Orsenigo G, Pacitti F, Roncone R, Santonastaso P, Siracusano A, Vignapiano A, Vita A, Zeppegno P, Maj M, . Interplay Among Psychopathologic Variables, Personal Resources, Context-Related Factors, and Real-life Functioning in Individuals With SchizophreniaA Network Analysis. JAMA Psychiatry. Published online February 14, 2018. doi:10.1001/jamapsychiatry.2017.4607
How are real-life functioning, psychopathologic variables, cognition, personal resources, and sociodemographic variables connected to each other in community-dwelling individuals with schizophrenia?
In this network analysis of 740 individuals with schizophrenia, functional capacity and everyday life skills were the most central and interconnected nodes, while positive symptoms were the least interconnected nodes. Real-life functioning was connected with several variables belonging to different domains.
The high centrality of functional capacity and everyday life skills suggests that improving the ability to perform tasks relevant to everyday life is critical for any therapeutic intervention in schizophrenia, and the pattern of network node connections supports the implementation of personalized interventions for individuals with schizophrenia.
Enhanced understanding of factors associated with symptomatic and functional recovery is instrumental to designing personalized treatment plans for people with schizophrenia. To date, this is the first study using network analysis to investigate the associations among cognitive, psychopathologic, and psychosocial variables in a large sample of community-dwelling individuals with schizophrenia.
To assess the interplay among psychopathologic variables, cognitive dysfunctions, functional capacity, personal resources, perceived stigma, and real-life functioning in individuals with schizophrenia, using a data-driven approach.
Design, Setting, and Participants
This multicenter, cross-sectional study involved 26 university psychiatric clinics and/or mental health departments. A total of 921 community-dwelling individuals with a DSM-IV diagnosis of schizophrenia who were stabilized on antipsychotic treatment were recruited from those consecutively presenting to the outpatient units of the sites between March 1, 2012, and September 30, 2013. Statistical analysis was conducted between July 1 and September 30, 2017.
Main Outcomes and Measures
Measures covered psychopathologic variables, neurocognition, social cognition, functional capacity, real-life functioning, resilience, perceived stigma, incentives, and service engagement.
Of 740 patients (221 women and 519 men; mean [SD] age, 40.0 [10.9] years) with complete data on the 27 study measures, 163 (22.0%) were remitted (with a score of mild or better on 8 core symptoms). The network analysis showed that functional capacity and everyday life skills were the most central and highly interconnected nodes in the network. Psychopathologic variables split in 2 domains, with positive symptoms being one of the most peripheral and least connected nodes. Functional capacity bridged cognition with everyday life skills; the everyday life skills node was connected to disorganization and expressive deficits. Interpersonal relationships and work skills were connected to avolition; the interpersonal relationships node was also linked to social competence, and the work skills node was linked to social incentives and engagement with mental health services. A case-dropping bootstrap procedure showed centrality indices correlations of 0.75 or greater between the original and randomly defined samples up to 481 of 740 case-dropping (65.0%). No difference in the network structure was found between men and women.
Conclusions and Relevance
The high centrality of functional capacity and everyday life skills in the network suggests that improving the ability to perform tasks relevant to everyday life is critical for any therapeutic intervention in schizophrenia. The pattern of network node connections supports the implementation of personalized interventions.
Schizophrenia is a severe mental disorder ranking among the top 20 leading causes of disability.1,2 Although the outcome of schizophrenia is not always as poor as believed in the past, functional recovery is observed in less than 15% of patients.3
Enhanced understanding of factors that hinder real-life functioning of people with schizophrenia is instrumental to design integrated and personalized treatment plans. To this aim, the Italian Network for Research on Psychoses has focused on the identification of variables influencing functioning, in particular, on the interplay among illness-related variables, personal resources, context-related variables, and real-life functioning.
However, modeling these associations and exploring their relative contribution to functioning by using structural equation models requires a priori assumptions concerning the associations among variables and the selection of predictors, mediators, and outcomes.4- 8 These assumptions may not always hold, as suggested by findings of nonunidirectionality of pathways leading to functioning: illness-related constructs may influence real-life functioning and vice versa.9- 11 In addition, structural equation models based on cross-sectional data do not allow simultaneous testing of reciprocal influences among predictors, mediators, and outcome measures.12
Network analysis represents an important innovation in the study of the interplay among variables. It is a data-driven approach that does not rely on an a priori model of cause-effect relationships among variables13,14 and produces spatially ordered networks in which key variables are located at the center of the network and variables with fewer connections at the periphery.14- 16 By inspecting a network, it is possible to understand the extent to which variables belonging to the same construct are connected and how different constructs are mutually interacting and reinforcing each other.
In this study, we applied network analysis to data collected on psychopathologic variables, neurocognition, functional capacity, personal resources, and functioning in a large sample of community-dwelling individuals with schizophrenia to gain a deeper insight into the complex mechanisms that associate these domains with each other.
Study participants were community-dwelling patients with schizophrenia who were stabilized on antipsychotic treatment and were seen consecutively at the outpatient units of 26 Italian university psychiatric clinics and/or mental health departments.8 Participants were recruited between March 1, 2012, and September 30, 2013. Inclusion and exclusion criteria are in eAppendix 1 in the Supplement. All patients provided written informed consent to participate after receiving a comprehensive explanation of study procedures and goals. The study protocol was approved by the local ethics committees of the participating centers (Campania University “Luigi Vanvitelli,” Naples; University of Turin, Turin; University of L’Aquila, L’Aquila; University of Bari, Bari; University of Catania, Catania; University of Foggia, Foggia; University of Genoa, Genoa; University of Cagliari, Cagliari; S. Andrea Hospital, Sapienza University of Rome, Rome; University of Siena, Siena; G. D’Annunzio University, Chieti; University of Pisa, Pisa; Sapienza University of Rome, Rome; University of Parma, Parma; University of Salerno, Salerno; University of Milan, Milan; University of L’Aquila, L’Aquila; University of Padua, Padua; Tor Vergata University of Rome, Rome; University of Brescia and Spedali Civili Hospital, Brescia; University of Eastern Piedmont, Novara; University of Bologna, Bologna; University of Florence, Florence; University of Naples Federico II, Naples; and University of Pisa, Pisa).
All measures used in the study are reported in Table 117- 29 and described in eAppendix 1 in the Supplement. Comparability of data collection procedures was assured by a centralized training of researchers, as reported in the supplemental document. A complete description of the study procedures is provided in the article by Galderisi et al.8
Statistical analysis was conducted between July 1 and September 30, 2017. Twenty-seven variables were selected for the network analyses (Table 2).
A network is a graphical representation of variables (nodes) and their correlations are depicted as edges (eFigure 1 in the Supplement). Thicker and more saturated edges represent stronger correlations, green edges represent positive correlations, and red edges represent negative correlations.
Different types of networks can be obtained: the association network uses zero-order correlations15 and provides a first general representation of the pairwise associations among variables. The zero-order correlation matrix of the variables is provided in the eTable in the Supplement. The concentration network is built on partial correlations, where the association between each pair of nodes is controlled for the influence of all the other variables.30 These correlations control for the shared variance between nodes and express the strength of the unique links connecting pairs of variables. The adaptive least absolute shrinkage and selection operator (LASSO) network14 is obtained by assigning penalties to partial correlations between variables to make small correlations automatically shrink to 0. Because the study variables were not normally distributed according to the Shapiro-Wilks test, we calculated nonparametric correlations using the nonparanormal transformation.31
The network display was based on the algorithm of Fruchterman and Reingold,32 which places strongly associated nodes at the center of the graph and weakly associated ones at the periphery. The algorithm determines the proximity of nodes by weighing the attractive and repulsive forces on each node, in analogy with forces regulating molecular or planetary equilibrium,32 such that nodes placed more closely are those that attract each other more. To further facilitate readability, we included in the network diagram only correlations of 0.05 or more.
To quantify the importance of each node in the adaptive LASSO network, we computed the centrality indices of betweenness, closeness, and strength.14,33 The betweenness of a node equals the number of times that it lies on the shortest path length between any 2 other nodes. Closeness indicates how easy it is to reach all other nodes from the node of interest and is computed as the inverse of the weighed sum of distances of a given node from all other nodes in the network.33 Nodes with high betweenness are those that facilitate connections in the network, while nodes with high closeness affect the other nodes more quickly or are more affected by the other nodes.14 Last, the node strength is the sum of the correlations of 1 node to all other nodes. For each index, high values reflect great centrality in the network, but high strength may also derive from very strong correlations between peripheral nodes belonging to the same domain. Centrality plots were created to represent these indices. As suggested by Epskamp et al34 the robustness of the network solution was assessed by estimating the accuracy of edge weights and the stability of centrality indices. Details on these analyses are provided in eAppendix 2 in the Supplement. Adaptive LASSO networks were also obtained for male and female patients separately, to test whether known sex-specific associations among variables were confirmed.
Data management and descriptive analyses were conducted using Stata, version 13.1 (StataCorp). We used R, version 3.3.3 (R Foundation for Statistical Computing) to perform the network analysis; specifically, the package qgraph35 was used to obtain the network and centrality indices; bootnet,34 to evaluate the network stability; and NetworkComparisonTest,36 to test network replicability in split samples as well as in men and women.
The study sample consisted of 740 patients with complete data on the 27 variables used to build the network. Comparison of patients with complete data vs the rest of the recruited sample (n = 181) revealed no significant differences in sex, age, educational level, and severity of illness. Thus, the subgroup of patients included in the network analysis can be considered to be representative of the overall sample. Demographic and clinical characteristics of included participants are reported in Table 3.
The adaptive LASSO network (Figure 1) showed that nodes belonging to each construct were generally strongly associated and close to each other and well separated from those belonging to different constructs. As shown in Figure 1, resilience, neurocognition, social cognition, and indices of real-life functioning formed spatially contiguous patterns, with densely interconnected nodes.
Only items regarding psychopathologic variables were not spatially contiguous: strong links were found between avolition and diminished expression, as well as between the positive and disorganization dimensions; however, connections between negative and positive and disorganization symptoms were weak or indirect. Still, psychopathologic variable nodes were proximal and inversely correlated with the real-life functioning nodes. Only the positive symptoms node was quite isolated, and it connected to the rest of the network only through its strong association with disorganization, which, besides its connection with everyday life skills, was linked to expressive deficit and functional capacity. Positive symptoms were also connected with service engagement (the higher the positive symptoms, the poorer the service engagement). Depression was connected with avolition, bridged avolition with resilience and stigma, was linked to the resilience node perception of self, and had no direct connection with positive and disorganization symptoms.
The neurocognition and social cognition constructs were adjacent and densely connected and interconnected. In the neurocognition domain, the working memory node showed the highest centrality: it was strongly connected to all the other neurocognition nodes and bridged them with social cognition and functional capacity and, through functional capacity, with real-life functioning nodes. Verbal learning, visuospatial learning, and attention were also directly connected with functional capacity. Visuospatial learning and processing speed were directly, although weakly, connected to The Awareness of Social Inference Test24 (TASIT) section 1: emotion recognition node (TASIT-1).
In the social cognition domain, the TASIT-1 node showed the highest betweenness and closeness (Figure 2) and, together with TASIT section 2: social inference minimal (TASIT-2), showed the highest strength. The TASIT-1 node was connected to all the other social cognition nodes and bridged the social cognition domain with the functional capacity node and, through the functional capacity node, with the real-life functioning nodes. The TASIT-1 node also showed an inverse association with service engagement, such that, the better the social cognition, the better the service engagement.
In the resilience domain, social competence was the node with the highest betweenness and closeness (Figure 2). It was associated with all the other resilience nodes and bridged the resilience domain with interpersonal functioning and stigma. It was also inversely and weakly associated with avolition. Perception of self was strongly connected to social competence, perception of future, and family cohesion; it was also inversely correlated with stigma and depression.
In the real-life functioning domain, everyday life skills had the highest centrality (Figure 2). It was strongly connected with the other 2 functioning nodes (interpersonal relationships and work skills) and with functional capacity. The everyday life skills node was also inversely correlated with the expressive deficit negative symptom construct, with the disorganization dimension, and with stigma.
The functional capacity and Specific Level of Functioning Scale (SLOF)25 everyday life skills were the most central nodes of the whole network (highest betweenness and closeness; Figure 2). As shown in Figure 1, functional capacity bridged neurocognition and social cognition with the everyday life skills node, which, in turn, was connected to the SLOF subscales Work and Interpersonal Relationships, to the expressive deficit negative node, and to the disorganization node.
Both the interpersonal relationships node and the work skills node were inversely associated with avolition (the more severe the avolition, the poorer the functioning), but the connection was stronger between the interpersonal relationships node and avolition. Poor work functioning was also associated with the availability of incentives and poor service engagement.
The LASSO networks for male and female participants did not differ significantly at NetworkComparisonTest36 permutation tests of global strength (network strength, 0.557; P = .62) and structural invariance (maximum difference between edges, 0.166; P = .40).
Replicability and robustness tests demonstrated the validity of the network solution (eAppendix 2 and eFigures 2-4 in the Supplement).
To our knowledge, this is the first study using a network approach to analyze the complex associations among psychopathologic variables, personal resources, context-related factors, and real-life functioning in a large sample of community-dwelling individuals with schizophrenia. Research studies performed to date using network analysis were focused on the interplay of symptoms in mood, anxiety, and eating disorders37 and only recently investigation has been extended to schizophrenia and the association of psychotic symptoms with environmental factors.38,39
Our study confirms that social cognition, neurocognition, resilience, and real-life functioning represent robust and independent constructs, with spatially contiguous and highly interconnected nodes, regardless of the use of 1 or more measures of the same construct. Psychopathologic variable nodes are the only exception: avolition, expressive deficit, and depression form a highly interconnected pattern on 1 side of real-life functioning nodes, densely connected with them and with the area of resilience and stigma. Positive symptoms and disorganization have few connections to the other nodes, as reflected by their low betweenness and closeness. In particular, positive symptoms show no direct connection with the real-life functioning nodes.
The study clearly shows that functional capacity and everyday life skills play a key role in the network: they have the highest centrality, as shown by their location in the network and their high betweenness and closeness. Functional capacity is the node that links neurocognition and social cognition with real-life functioning nodes, in particular with everyday life skills, such as household activities, handling of personal finances, and use of the telephone or public transportation. The greater proximity of functional capacity to everyday functioning with respect to neuropsychological measures6,40,41 supports the choice of functional capacity as a functionally meaningful coprimary measure in clinical trials of cognition enhancement in schizophrenia.42
According to the network analysis, and in line with previous reports,43,44 social cognition is highly interconnected with neurocognition, and both nodes show a strong link with functional capacity and, through functional capacity, with real-life functioning. The social cognition node directly connected to functional capacity is TASIT-1, which measures the ability to identify basic emotions in a series of brief videotaped vignettes, in which professional actors produce spontaneous emotional displays resembling those encountered in everyday social interactions.24
The connection between recognition of emotions and functional capacity highlights the role of social and emotional processing in general psychosocial functioning. As pointed out by Thaler et al,44 individuals who are able to comprehend social and emotional stimuli may have acquired better interpersonal skills required for some of the tasks incorporated in the functional capacity assessment (eg, communication skills).
Our network analysis shows that social competence, not social cognition, is directly linked to interpersonal functioning. This connection might be the result of overlapping constructs in the 2 instruments. However, social competence is self-rated, while social cognition is based on caregiver reports.
Our network analysis did not show direct connections of social cognition with real-life functioning nodes, which is at odds with some previous reports.4,8,45 However, those reports used structural equation models and modeled social cognition as a mediator of neurocognitive effects on functioning. Our results cannot directly address the issue of mediation effects, as network analysis does not have a consolidated procedure to test these effects; however, they suggest that functional capacity bridges social cognition and real-life functioning.
The other key variable of the network is the domain of everyday life skills. This domain shows the highest closeness and betweenness centralities: it links real-life functioning with psychopathologic variables and functional capacity, which, in turn, connects everyday life skills to the cognition nodes. The everyday life skills are also connected, although weakly, with internalized stigma.
The other 2 real-life functioning nodes (ie, interpersonal relationships and work skills) show a lower centrality in the network. As noted by Leifker et al,46 different domains of everyday functioning have different determinants. Although this finding could not be tested in a previous study8 based on a structural equation model in which the SLOF subscales were combined into a latent variable, the network approach clearly shows that cognition and functional capacity are connected to everyday life skills but not to interpersonal functioning. On the contrary, avolition is inversely connected to interpersonal functioning and weakly connected to work skills but not to everyday life skills. This is in line with previous studies showing a significant association between SLOF interpersonal relationships and avolition, both in individuals with schizophrenia46,47 and their first-degree nonaffected relatives.48
Our findings also support the discriminant validity of the 2 negative symptom factors, which, although strongly connected to each other, do show a different pattern of connections. Specifically, avolition is connected to depression and social competence, as well as to SLOF interpersonal relationships and work skills, while expressive deficit is connected with disorganization, functional capacity, and everyday life skills. Interpersonal functioning is also connected to social competence, which is actually the only node bridging resilience and real-life functioning, besides the weak connection between internalized stigma and everyday life skills.
In line with studies by Galderisi et al8 and Strassnig et al,41 our results confirm that depression has no direct influence on real-life functioning. Specifically, depression is connected to resilience, self-stigma, and avolition and only indirectly connected, through self-stigma and avolition, to the 3 nodes of functioning.
Our results might have substantial implications for treatment if they are confirmed in longitudinal studies in which the direction of effects can be tested. The high centrality of functional capacity and everyday life skills in the network is in line with the recovery-oriented approaches to schizophrenia, maintaining that the ability to perform tasks relevant to everyday life should represent the primary target of rehabilitation programs.49- 52 The cross-sectional nature of our findings does not allow firm conclusions, as the centrality measures may be high because the nodes strongly influence the rest of the system but also because they are influenced by other nodes.
In spite of their cross-sectional nature, our findings strongly suggest that treatment beyond antipsychotic drugs is needed for people with schizophrenia, as positive symptoms are a peripheral node in the network. Therefore, targeting positive symptoms only, as treatment with antipsychotic medication does, is unlikely to lead to recovery in adults with schizophrenia.
In addition, the pattern of connections among the network nodes indicates that, depending on the patient’s characteristics, different rehabilitation programs should be implemented. For instance, targeting the cognition area may affect functional capacity more strongly and, with cascading effects, may improve functioning in everyday life activities and, indirectly and to a minor extent, work skills and interpersonal relationships. This finding is in line with meta-analyses of rehabilitation interventions, showing that treatment of cognitive deficits should be combined with interventions aimed at ameliorating skills deficits to yield significant functional benefits.53
The main strength of our study is the use of the network approach to investigate the pattern of connections among a wide array of cognitive, psychopathologic, and psychosocial variables from a large sample of community-dwelling individuals with schizophrenia who are stabilized on antipsychotic treatment (mainly second-generation antipsychotic medications).
The network analysis might contribute to the shift from simpler, a priori models of established causal associations between variables to the recognition of the complex interdependence between disease-related factors, personal resources, environmental variables, and real-life functioning. This shift might be instrumental in promoting a personalized and integrated approach to the treatment of schizophrenia.
Some limitations of the study must be acknowledged. First, the cross-sectional nature of the study does not allow appropriate testing of the direction of the effects. Second, owing to the inclusion of only patients with established schizophrenia who are clinically stable, in whom the effect of positive symptoms and disorganization on functioning might be less central than in patients with a first episode of schizophrenia, our results might not generalize to the latter patient population. Third, a further limitation is represented by the fact that the effects of antipsychotics and of self-report vs interview-based instruments on the network structure cannot be estimated. Finally, the network analysis, at odds with the structural equation model, cannot address the issue of mediation effects, as it does not have a consolidated procedure to test these effects.
The high centrality of functional capacity and everyday life skills in the network suggests that improving the ability to perform tasks relevant to everyday life is critical for any therapeutic intervention in individuals with schizophrenia. The pattern of network node connections supports the implementation of personalized interventions.
Accepted for Publication: December 17, 2017.
Corresponding author: Silvana Galderisi, MD, Department of Psychiatry, Campania University “Luigi Vanvitelli,” Largo Madonna delle Grazie, 80138 Naples, Italy (email@example.com).
Published Online: February 14, 2018. doi:10.1001/jamapsychiatry.2017.4607
Author Contributions: Drs Galderisi and Rucci had full access to all the data in the study and take responsibility for the integrity of the data and the accuracy of the data analysis.
Study concept and design: Galderisi, Mucci, Maj.
Acquisition, analysis, or interpretation of data: Galderisi, Rucci, Kirkpatrick, Gibertoni, Rocca, Rossi, Bertolino, Strauss, Aguglia, Bellomo, Murri, Bucci, Carpiniello, Comparelli, Cuomo, De Berardis, Dell’Osso, Di Fabio, Gelao, Marchesi, Monteleone, Montemagni, Orsenigo, Pacitti, Roncone, Santonastaso, Siracusano, Vignapiano, Vita, Zeppegno, Maj.
Drafting of the manuscript: Galderisi, Rucci, Mucci, Gibertoni, Strauss, Maj.
Critical revision of the manuscript for important intellectual content: Galderisi, Kirkpatrick, Mucci, Gibertoni, Rocca, Rossi, Bertolino, Strauss, Aguglia, Bellomo, Murri, Bucci, Carpiniello, Comparelli, Cuomo, De Berardis, Dell’Osso, Di Fabio, Gelao, Marchesi, Monteleone, Montemagni, Orsenigo, Pacitti, Roncone, Santonastaso, Siracusano, Vignapiano, Vita, Zeppegno, Maj.
Statistical analysis: Rucci, Gibertoni.
Obtained funding: Maj.
Administrative, technical, or material support: Maj.
Study supervision: Galderisi, Maj.
Conflict of Interest Disclosures: Dr Kirkpatrick reported receiving licensing royalties from ProPhase LLC for use of the Brief Negative Symptom Scale (BNSS) by for-profit groups (these fees are donated to the Brain and Behavior Research Foundation); receiving honoraria and travel support from ProPhase LLC for training pharmaceutical company raters on the BNSS, consulting fees and/or travel support from Genentech/Roche, Minerva Neurosciences, ProPhase LLC, and Allergan, consulting fees from anonymized pharmaceutical companies through Decision Resources Inc and from an anonymized investment capital company through Guideposts; working with Allergan to prepare a manuscript without receiving a fee; receiving fees from Walsh Medical Media and from Physicians Postgraduate Press Inc for editorial services. No other conflicts were reported.
Funding/Support: The study was funded by the Italian Ministry of Education, the Italian Society of Psychopathology, the Italian Society of Biological Psychiatry, Roche, Lilly, AstraZeneca, Lundbeck, and Bristol-Myers Squibb.
Role of the Funder/Sponsor: The funding sources had no role in the design and conduct of the study; collection, management, analysis, and interpretation of the data; preparation, review, or approval of the manuscript; and decision to submit the manuscript for publication.
Group Information: The following members of the Italian Network for Research on Psychoses contributed to the recruitment of patients; clinical, neuropsychological, and functional assessments; and data quality control: Carmen Aiello, MD, Debora Molle, MD, Alessia Nicita, MD, Sara Patriarca, MD, and Daria Pietrafesa, MD (Department of Psychiatry, Campania University “Luigi Vanvitelli,” Naples, Italy); Luisa Longo, MD, Andrea Falsetti, MD, and Marina Barone, MD (Department of Neurological and Psychiatric Sciences, University of Bari, Bari, Italy); Alessandro Galluzzo, MD, Stefano Barlati, MD, and Giacomo Deste, MD (Psychiatric Unit, School of Medicine, University of Brescia, and Department of Mental Health, Spedali Civili Hospital, Brescia, Italy); Federica Pinna, MD, Diego Primavera, MD, and Lucia Sanna, MD (Department of Public Health, Clinical and Molecular Medicine, Section of Psychiatry, University of Cagliari, Cagliari, Italy); Maria Salvina Signorelli, MD, Giuseppe Minutolo, MD, and Dario Cannavò, MD (Department of Clinical and Molecular Biomedicine, Psychiatry Unit, University of Catania, Catania, Italy); Mariangela Corbo, MD, Gaia Baroni, MD, and Chiara Montemitro, MD, (Department of Neuroscience and Imaging, G. D’Annunzio University, Chieti, Italy); Mario Altamura, MD, Maddalena La Montagna, MD, and Raffaella Carnevale, MD (Department of Medical Sciences, Psychiatry Unit, University of Foggia, Foggia, Italy); Mario Amore, MD, Pietro Calcagno, MD, and Michele Bugliani, MD (Department of Neurosciences, Rehabilitation, Ophthalmology, Genetics and Maternal and Child Health, Section of Psychiatry, University of Genoa, Genoa, Italy); Serena Parnanzone, MD, Rodolfo Rossi, MD, and Dario Serrone, MD (Department of Biotechnological and Applied Clinical Sciences, Section of Psychiatry, University of L’Aquila, L’Aquila, Italy); Laura Giusti, PhD, Maurizio Malavolta, MD, and Anna Salza, PhD (Department of Life, Health and Environmental Sciences, Unit of Psychiatry, University of L’Aquila, L’Aquila, Italy); Alice Caldiroli, MD, Gianmario Mandolini, MD, and Vera De Carlo, MD (Department of Psychiatry, University of Milan, Milan, Italy); Andrea de Bartolomeis, MD (Department of Neuroscience, Section of Psychiatry, University of Naples Federico II, Naples, Italy); Carla Gramaglia, MD, Debora Marangon, MD, and Pierluigi Prosperini, MD, (Department of Translational Medicine, Psychiatric Unit, University of Eastern Piedmont, Novara, Italy); Elena Tenconi, PhD, Paolo Meneguzzo, MD, and Valeria Giannunzio, MD (Psychiatric Clinic, Department of Neurosciences, University of Padua, Padua, Italy); Matteo Tonna, MD, Paolo Ossola, MD, and Maria Lidia Gerra, MD (Department of Neuroscience, Psychiatry Unit, University of Parma, Parma, Italy); Camilla Gesi, MD, Ivan Mirko Cremone, MD, and Barbara Carpita, MD (Department of Clinical and Experimental Medicine, Section of Psychiatry, University of Pisa, Pisa, Italy); Roberto Brugnoli, MD, Antonio Del Casale, MD, and Valentina Corigliano, MD (Department of Neurosciences, Mental Health and Sensory Organs, S. Andrea Hospital, Sapienza University of Rome, Rome, Italy); Massimo Biondi, MD, Monica Zocconali, PhD, and Antonino Buzzanca, PhD (Department of Neurology and Psychiatry, Sapienza University of Rome, Rome, Italy); Giulio Corrivetti, MD, Gaetano Pinto, MD, and Ferdinando Diasco, MD (Department of Medicine, Surgery and Dentistry “Scuola Medica Salernitana,” Section of Neuroscience, University of Salerno, Salerno, Italy); Andrea Fagiolini, MD, Arianna Goracci, MD, and Simone Bolognesi, PhD (Department of Molecular Medicine and Clinical Department of Mental Health, University of Siena, Siena, Italy); Cinzia Niolu, MD, Giorgio Di Lorenzo, MD, and Michele Ribolsi, MD (Department of Systems Medicine, Psychiatry and Clinical Psychology Unit, Tor Vergata University of Rome, Rome, Italy); and Irene Mancini, MD, Claudio Brasso, MD, and Paola Bozzatello, MD (Department of Neuroscience, Section of Psychiatry, University of Turin, Turin, Italy).