We have shown that a dye such as eosin or mercurochrome when injected either intravenously or intravesicularly will visualize the gallbladder and extrahepatic biliary ducts in the opened abdomen of rabbits when they are exposed to filtered ultraviolet radiation.1 In our work the source of ultraviolet radiation was a mercury vapor arc lamp with a nickel oxide filter. The filter permits the selection of waves ranging from 300 to 400 millimicrons in length (with the greatest spectral intensity at 366 millimicrons). Tissues or fluorescent dyes when examined by filtered ultraviolet radiation emit secondary visible rays; this phenomenon is called fluorescence.2 In the aforementioned investigation we found that the dye, which fluoresces as a golden color, can be seen progressing from the gallbladder to the duodenum, and then farther down the intestinal tract (figs. 1 and 2). Obstruction of either the biliary or the intestinal tract can be demonstrated
SUTRO CJ, BURMAN MS. EXAMINATION OF TUBULAR ORGANS AND ARTERIAL SYSTEMS OF RABBITS BY FILTERED ULTRAVIOLET RADIATION. Arch Surg. 1934;28(5):867–873. doi:10.1001/archsurg.1934.01170170060005
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