Two methods can be used for perfusing small animals. Either the heart and lung of a second animal can be used as pump and oxygenator or a small mechanical oxygenator can be used.
With a mechanical pump oxygenator, the liver, lung, and spleen can be excluded in the perfusion of an extremity, for example. The exclusion of these organs is particularly helpful for a study of the perfusion of colloids, since these organs can remove some colloids in a single passage of blood.1
For a research program on chemically modified antibodies for cancer therapy, antibody complexes with a variety of radioactive and nonradioactive colloids are being investigated. The cytotoxic colloids being used include the fissionable actinides and boron-10 for the neutron capture therapy of tumors2; β and γ emittors; magnetic particles such as iron oxide for inductive heating of tumors, and suitably insolubilized antimetabolites and chelating agents. In
KNOCK FE. Disposable Pump Oxygenator for Perfusion in Small Animals: Used in Perfusing Chemically Modified Antibodies. Arch Surg. 1960;81(4):668–670. doi:https://doi.org/10.1001/archsurg.1960.01300040152029
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