[Skip to Navigation]
Access to paid content on this site is currently suspended due to excessive activity being detected from your IP address Please contact the publisher to request reinstatement.
March 1961

Hypothermia Against Lethal Dose of Mechlorethamine: Protective Effect in Dogs

Author Affiliations

From the Department of Surgery, Duke University Medical Center, and the Department of Pathology, Duke University Medical Center and Veterans Administration Hospital, Durham, N.C.

Arch Surg. 1961;82(3):400-404. doi:10.1001/archsurg.1961.01300090070014

One of the problems involved in regional perfusion for cancer chemotherapy is protection of the bone marrow outside of the perfused area due to "leak" of the antitumor agent from the perfused area. Since in vitro experiments have shown that reduction in incubation temperature below 37 C depresses the antitumor effect of certain alkylating agents against experimental animal tumors,4 it became desirable to determine if reduction of body temperature would protect the hematopoietic system of the animal against lethal effects of an alkylating agent.

Method  Paired, healthy mongrel dogs were lightly anesthetized with pentobarbital (Nembutal) anesthesia. The body temperature of one of the paired animals was reduced from 37 C to 31-33 C by immersion in an ice-water bath, and the animal was then removed and allowed to rewarm spontaneously at room temperature of approximately 27 C. A 3-4 cm. segment of rib from each of the 2 animals