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November 1961

Cardiac Storage with Glycerol at Zero Centigrade

Author Affiliations

From the Department of Surgery, University Medical Center. This work was supported in part by grants from the Mississippi Heart Association and National Institutes of Health (H-2806).

Arch Surg. 1961;83(5):719-720. doi:10.1001/archsurg.1961.01300170075014

At present, the only long-term storage method for tissues and organs is freezing with glycerol. Much work has been done in establishing low-temperature storage norms for tissues such as spermatozoa,6,7 blood,3,8 bone,5 arteries,4 skin,2 and endocrine organs.9 Little or no successful long-term cold storage work has been done with the mammalian heart, though freezing down to almost absolute zero has been accomplished with transient recovery.1 In the studies and results obtained and herein reported, rats' hearts were successfully stored in glycerol at temperatures ranging from 25 C to 0 C.

Method  Rats were anesthetized with pentobarbital, injected with 0.25 mg. of heparin,10 and exsanguinated by severing the abdominal aorta. The rib cage was opened, and the intact heart, lungs, and aorta were excised. A cannula made from a blunted 18-gauge needle was tied into the aorta with its tip just above the

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