There have been several reports on the effectiveness of protamine and hexadimethrine (Polybrene) in neutralizing the effects of heparin. Parkin and Kvale1 showed in rabbits with no extracorporeal circulation that clotting times returned to normal at a 1.5:1 dose of protamine to heparin in 5 minutes. In 1953, Preston and Parker2 reported on the effects of hexadimethrine on the clotting time in peptone shock and in experimental radiation injury. They found that in the normal animal 7.5 mg. hexadimethrine neutralized 10 mg. of heparin in vivo. In peptone shock, when the coagulation time was prolonged to 56 minutes or more, hexadimethrine in doses of 0.9 to 2.2 mg. per kilogram restored the coagulation time to approximately control levels within a few minutes. In vitro studies showed that the shortest clotting time occurred when the amount of protamine and heparin exactly neutralized each other. If either was in excess,
GOLDMAN R, HOHF RP. Hexadimethrine and Protamine as Antiheparin Agents: An Experimental Comparison After Total-Body Perfusion in Dogs. Arch Surg. 1962;85(3):497–502. doi:10.1001/archsurg.1962.01310030145023
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