[Skip to Content]
[Skip to Content Landing]
February 1963

Transplantation of Autologous Canine Thyroid: Transplantation After Preservation at Low Temperature

Author Affiliations

John and Mary R. Markle Scholar in Medical Science, U.S. Public Health Service Career Development Awardee, AM-K3, 13,702 (Dr. Zuidema).

Arch Surg. 1963;86(2):246-249. doi:10.1001/archsurg.1963.01310080070016

This experiment was undertaken to develop a method of in vitro preservation of tissue in a viable state. Skin, bone, dura mater, and blood vessels have been transplanted successfully after periods of in vitro storage, but these tissues are usually preserved in a nonviable state and have been selected more for their structural or physical properties than for their physiological function. Tissue culture techniques have, of course, been highly developed, and strains of normal and neoplastic cells have been maintained for long periods of time. Certain aspects of tissue culture work have been applied to this problem in an effort to preserve thyroid tissue in vitro at low temperature and to demonstrate its viability upon reimplantation in the donor animal.

Thyroid tissue was selected because viability could be demonstrated by its uptake of radioactive iodine as well as by histological study. Endocrine tissue is well suited for transplantation because it