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September 1964

The Glucocorticoid Deprived Myocardium: Enzymatic and Electron Microscopic Studies

Author Affiliations

Post doctoral research fellow of the National Heart Institute (Dr. Lockwood).; From the Department of Surgery and Medicine, University of Mississippi School of Medicine, Jackson, Miss, and the Department of Surgery, University of Texas Southwestern Medical School.

Arch Surg. 1964;89(3):457-461. doi:10.1001/archsurg.1964.01320030047007

In previous investigations we have oberved that the glucocorticoid restricted myocardium responds as if in failure both functionally and metabolically.1 Others2,3 have shown that glucocorticoids act as a myocardial protective substance in the isolated heart-lung preparation. Tanz3 observed marked myocardial degeneration in the perfused heart after 6 hours of continuous stimulation, but when cortisone was added the myocardium maintained good cellular integrity.

These observations led us to study the micro-anatomy and the enzymatic alterations of the heart after adrenalectomy.

Methods  Twelve healthy mongrel dogs, weighing 10 to 29 kg, were prepared with a single-stage trans-abdominal bilateral adrenalectomy and maintained on 2.5 mg desoxycorticosterone acetate (Doca acetate) daily and additional NaCl. During the first five days decreasing amounts of hydrocortisone were given twice daily. After the fifth postoperative day hydrocortisone was discontinued. Daily blood samples were drawn for serum glutamic oxalacetic transaminase (SGOT) and serum glutamic pyruvic transaminase