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February 1969

Induced Hemorrhagic Hypotension: Its Effect on Plasma 5-Hydroxytryptamine and Plasma 5-Hydroxyindole Acetic Acid Levels

Author Affiliations

Brooklyn, NY
From the departments of surgery and psychiatry, State University of New York Downstate Medical Center, Brooklyn, NY. Dr. Schenker is now at the Veterans Administration Hospital, Albany, NY, and Dr. Stuckey is a career scientist from the Health Research Council of New York.

Arch Surg. 1969;98(2):194-198. doi:10.1001/archsurg.1969.01340080086017

The effect of hemorrhagic hypotension on plasma serotonin levels has been investigated previously,1,2 but it remains to be shown conclusively whether or not the level of this biogenic amine rises, falls, or remains constant as a result of hypotension. Since serotonin (5-HT) is rapidly metabolized to 5-hydroxyindole acetic acid (5-HIAA), changes in plasma 5-HT are reflected by changes in the plasma 5-HIAA levels.3,4 In the present study, simultaneous determinations of plasma 5-HT and of plasma 5-HIAA, the predominant end-product of 5-HT metabolism, are made at specific intervals before and during hypotension induced by hemorrhage in an attempt to contribute further information on this subject.

Methods and Materials  Reagent grade chemicals and solvents were used in the serotonin determinations made by a method modified from Waalkes.5 These included the following: (1) NaCl; (2) n-butyl alcohol; (3) n-heptane (commercial n-heptane washed with equal volumes of 0.1