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September 1969

Short-Term in Situ Perfusion of the Canine Kidney Following Death

Author Affiliations

Los Angeles
From the Surgical Service, Wadsworth Hospital, Veterans Administration Center, and Department of Surgery, University of California Medical Center, Los Angeles.

Arch Surg. 1969;99(3):413-417. doi:10.1001/archsurg.1969.01340150121027

Experiments have been done to determine if isolated in situ perfusion of the cadaver kidney will preserve renal viability. Perfusion experiments were performed under varying conditions of time, pressure, renal blood flow, and temperature. The tetrazolium bromide dye reduction time, histology, and radioisotope renograms were all used as an index of kidney viability.

Materials and Methods  Isolated in situ perfusion of the kid[ill] neys was performed in 22 healthy mongrel dogs of both sexes weighing 15 to 30 kg (33 to 66 lb). Pentobarbital sodium was given intravenously in doses of 30 mg/kg for anesthesia. Immediately prior to perfusion each dog was killed with a lethal dose of pentobarbital. The pump perfusate was autologous whole blood obtained from the external jugular or femoral vein. It was oxygenated with a disposable Bentley oxygenator (oxygen [O2] flow at 3 liters/min), and passed through a blood administration set filter. The oxygenated, diluted