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October 1971

Microscopic and Ultrastructural Changes in Unmodified Canine Lung Allografts

Author Affiliations

From the Department of Surgery, Harvard Medical School, and the Cardiovascular Division, Sears Surgical Research Laboratories, Harvard Unit, Boston City Hospital.

Arch Surg. 1971;103(4):490-495. doi:10.1001/archsurg.1971.01350100088016

Left lung allografts were performed in ten mongrel dogs. No immunosuppressives were given. Serial biopsies of the transplanted lungs were excised for light and electron microscopy before and after transplantation. All biopsies for electron microscopy were taken in the inflated state. Alveolar surfactant activity was determined on a portion by the indirect bubble stability method and the remainder was fixed for light (toluidine blue) and ultrastructural examination. Ultrastructural alterations in the homografted lungs were noted as early as the first day. These changes occurred earlier than previously described histologic characterizations of the pulmonary allograft rejection sequences. Correlations of the morphologic findings with surfactant activity determinations could not be made until rejection was advanced.