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June 1988

Abnormal Rabbit Heterophil Chemotaxis Following Thermal Injury: An In Vivo Model of an Abnormality of the Chemoattractant Receptor for f-met-leu-phe

Author Affiliations

From the Department of Surgery, Cornell University Medical College, New York (Dr Davis); and the Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Washington, DC (Dr Gallin).

Arch Surg. 1988;123(6):752-755. doi:10.1001/archsurg.1988.01400300098017

• Previous studies have shown that the decreased neutrophil migratory responsiveness seen in burned patients correlates with the extent of thermal injury and the extent of the neutrophil-specific granule deficiency. To understand better the relationship between the neutrophil dysfunction, degranulation, and thermal injury, a rabbit model was studied. Eighteen rabbits were burned over 20% of their surface area. Assay of peripheral blood heterophils disclosed decreased migratory activity compared with preburn levels and decreased lysozyme content vs preburn levels, but no change in the β-glucuronidase content. The specific binding of tritiated formyl-methionyl-leucyl-phenylalanine to peripheral blood heterophils was increased fivefold over that of control cells. These studies indicate that, following thermal injury, there is a selective decrease of specific granule contents and an increase in chemoattractant binding to the cell and also suggest an abnormality in chemoattractant receptor processing. The rabbit provides a convenient model for the study of compromised host defenses following thermal injury.

(Arch Surg 1988;123:752-755)

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