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August 1993

Effect of Lithium on Incorporation of Bromodeoxyuridine and Tritiated Thymidine Into Human Parathyroid Cells

Author Affiliations

From the Department of Surgery and the DeRoy Surgical Research Laboratory, Sinai Hospital, Detroit, Mich.

Arch Surg. 1993;128(8):865-869. doi:10.1001/archsurg.1993.01420200039007

Objective:  To measure lithium chloride—stimulated incorporation of tritiated thymidine (3H-TdR) and bromodeoxyuridine (BrdU) by human parathyroid cells as an indicator of lithium's growth-promoting properties.

Design:  Cell suspensions derived from abnormal human parathyroid tissue were incubated for 5 days with graded concentrations of lithium and pulsed with either 0.074 MBq of 3H-TdR or 0.6 μg of BrdU. The 3H-TdR incorporation was measured by counting cell filtrates in a beta counter, and nuclear BrdU incorporation was determined by counting nuclei positively stained by an antibody to BrdU. Counts of BrdU-positive cells by two observers unaware of incubation conditions were correlated with 3H-TdR counts.

Results:  Cell suspensions derived from adenomatous and hyperplastic parathyroid glands demonstrated a dose-related increase in 3H-TdR incorporation. There was close correlation between the two observers' counts of BrdU-positive cells (r=0.70, P=.01) and between each observer's counts and 3H-TdR counts (r=0.70, P=.02, and r=0.90, P=.01, respectively).

Conclusions:  Lithium stimulates in vitro incorporation of 3H-TdR and BrdU by abnormal human parathyroid cells, consistent with a growth-promoting effect.(Arch Surg. 1993;128:865-869)

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