Effect of hypertonic saline infusion on liver function after lower-torso ischemia-reperfusion and 2 hours of reperfusion. Error bars represent SEM. Asterisk indicates P<.05 vs isotonic sodium chloride solution.
Hypertonic saline administration results in marked diminution of mean serum levels of tumor necrosis factor α (TNF-α) after reperfusion. Error bars represent SEM. Asterisk indicates P<.05 vs isotonic sodium chloride solution.
Mean interleukin 6 (IL-6) secretion subsequent to ischemia-reperfusion injury was significantly diminished after exposure to hypertonic saline. Error bars represent SEM. Asterisk indicates P<.05 vs isotonic sodium chloride solution.
Photomicrographs of lung tissue after 2 hours of reperfusion (original magnification ×100). A, Control lung exhibiting normal architecture. B, Isotonic sodium chloride solution groups showing pronounced acute inflammatory infiltrate with destruction of parenchyma, hemorrhage, edema, and alveolar collapse. C and D, Hypertonic saline (pretreatment) and hypertonic saline groups, respectively, showing congestion and a moderate infiltration of acute inflammatory cells.
Improved survival is seen after resuscitation with hypertonic saline. Isotonic sodium chloride solution (4 or 30 mL/kg) or hypertonic saline (4 mL/kg) was administered after induction of lower-torso ischemia-reperfusion injury. P<.05 vs the isotonic sodium chloride solution groups.
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Shields CJ, Winter DC, Manning BJ, Wang JH, Kirwan WO, Redmond HP. Hypertonic Saline Infusion for Pulmonary Injury Due to Ischemia-Reperfusion. Arch Surg. 2003;138(1):9–14. doi:10.1001/archsurg.138.1.9
Inhibition of neutrophil-endothelial cell interactions by hypertonic saline (HTS) may confer protection against organ injury in states of immunologic disarray. This study tested the hypothesis that infusion of HTS modulates the development of end-organ injury in a model of lower-torso ischemia-reperfusion injury.
Ischemia-reperfusion injury was induced in 30 male Sprague-Dawley rats by infrarenal aortic cross-clamp for 30 minutes, followed by reperfusion for 2 hours. At 0 and 60 minutes of reperfusion, intravenous HTS (7.5% sodium chloride, 4 mL/kg) was administered to 6 rats each, and another 12 received either 4 or 30 mL/kg of isotonic sodium chloride solution. Six rats received HTS, 4 mL/kg, before ischemia. At 2 hours, we assessed liver function, pulmonary injury, neutrophil infiltration (myeloperoxidase activity), endothelial permeability (bronchoalveolar lavage and wet-dry weight ratios), and proinflammatory cytokine levels (tumor necrosis factor α and interleukin 6).
Infusion with HTS before or after ischemia significantly reduced end-organ injury. Histopathologic pulmonary injury scores were markedly attenuated in the HTS group (5.82 ± 1.3) and the HTS pretreated group (4.91 ± 1.6) compared with the isotonic sodium chloride solution groups (8.54 ± 1.1) (P = .04). Pulmonary neutrophil sequestration (2.07 ± 0.23) and increased endothelial permeability (4.68 ± 0.44) were manifest in animals resuscitated with isotonic sodium chloride solution compared with HTS treatment (1.54 ± 0.19 [P = .04] and 2.06 ± 0.26 [P = .02]) and pretreatment (1.18 ± 0.12 [P = .04] and 1.25 ± 0.07 [P = .002]). In addition, a significant reduction in serum tumor necrosis factor α (P = .04) and interleukin 6 (P = .048) levels was observed, whereas HTS resuscitation attenuated the upsurge in aspartate transaminase (P = .03) and alanine transaminase levels (P = .047).
Resuscitation with HTS attenuates the pulmonary edema and tissue injury due to lower-torso ischemia-reperfusion and maintains a more benign immunologic profile.
AN EPISODE OF acute lower-torso ischemia followed by restitution of perfusion may engender a diffuse and potentially life-threatening systemic inflammatory response,1-3 of which lung injury is the most pertinent manifestation.4 The propensity of an extrapulmonary pathologic condition to induce an acute response in the lung is predicated on the complex interplay of proinflammatory and anti-inflammatory factors.5 Although the mechanisms that initiate progression to end-organ injury remain ill defined,6-8 excessive neutrophil cytotoxicity is compellingly implicated in microvascular hyperpermeability, with spillage of protein-rich transudate into the alveolar spaces (hallmarks of the acute respiratory distress syndrome).9,10 The pivotal importance of neutrophil accumulation in the instigation of pulmonary injury is substantiated by the findings of studies using agents to abrogate neutrophil cytotoxicity.11 Teleologically, the cytotoxic potential of neutrophils equips the host to combat septic challenge. However, in states of immunologic disarray, as prevails in systemic inflammatory response syndrome, the unleashing of their destructive faculties results in tissue damage.11
The mediation of neutrophil entrapment in the lung is a consequence of complement activation, cytokine production, and stimulation of alveolar macrophages and adhesion molecule expression.12 Secretion of chemoattractant substances, including tumor necrosis factor α (TNF-α), interleukin 1β (IL-1β), and complement factor C5a, results in enticement of neutrophils to the pulmonary microvasculature.13,14 Pulmonary infiltration of activated neutrophils and their ultimate degranulation of proteolytic enzymes and reactive oxygen species results in the stigmata of lung injury.15 This manifests clinically as progressive hypoxemia with radiologic evidence of diffuse infiltrations, and it has been described in patients after aortic aneurysm repair.16-18
The immunomodulatory effects of hypertonic saline (HTS) infusion provide potential strategies for attenuating inappropriate neutrophil activation. Previous studies by Shields et al4,19 have demonstrated significant attenuation of end-organ injury in an animal model of pancreatitis with HTS resuscitation. The benefits of transient hyperosmolar resuscitation extend to the attenuation of receptor-mediated polymorphonuclear leukocyte functions, including the down-regulation of neutrophil oxidative burst activity and adhesion molecule expression and the suppression of polymorphonuclear leukocyte activation.20-22 The inhibition of neutrophil-endothelial cell interactions by HTS infusion may confer protection against organ injury in inappropriate inflammatory states.
This study tests the hypothesis that HTS infusion modulates the immunologic profile and development of end-organ injury induced by lower-torso ischemia-reperfusion (IR) in an animal model.
A neutrophil-mediated lung injury was induced using a model of aortic occlusion and revascularization described previously.7 Male Sprague-Dawley rats (weight, 250-350 g) were randomized into 5 groups. Anesthesia was induced with intraperitoneal thiopental sodium and was maintained with inhalational halothane. All animals underwent a midline laparotomy, followed by systemic heparinization (heparin sodium, 400 U/kg). Control animals sustained a sham IR injury, with exposure of the aorta, and all other animals underwent infrarenal aortic occlusion with a microvascular clamp for 30 minutes, followed by reperfusion for 2 hours. All salts, drugs, and chemicals were purchased from Sigma-Aldrich Co (St Louis, Mo).
At the time of reperfusion, resuscitation was performed on all animals. Control animals and those in the small-volume isotonic sodium chloride solution resuscitation group received a bolus of isotonic sodium chloride solution (0.9% sodium chloride, 4 mL/kg) via tail vein cannulation. This bolus was repeated at 1 hour of reperfusion. In an analogous manner, rats in the HTS and HTS pretreatment groups received 4 mL/kg of 7.5% sodium chloride at the same times. Hypertonic saline pretreatment animals had received an intravenous bolus of HTS (4 mL/kg) before the induction of ischemia. To control for alterations in microvascular flow properties and volume replacement, another group received large-volume isotonic sodium chloride solution resuscitation (0.9% sodium chloride, 30 mL/kg). Samples from control animals were used to provide baseline biochemical and histopathologic variables. All animals were humanely killed at the conclusion of 2 hours of reperfusion. This experiment was conducted after receiving approval by the Biological Services Unit of the National University of Ireland and the Department of Health and Children in Ireland (Dublin).
Immediately before euthanasia, blood samples were collected via cardiac puncture for biochemical determination of liver function. Plasma levels of aspartate transaminase and alanine transaminase were assessed, and serum levels of the cytokines TNF-α and IL-6 were determined using an enzyme-linked immunosorbent assay kit (R & D Systems, Minneapolis, Minn) and were estimated spectrophotometrically (MRX Microplate Reader; Dynatech Technologies Inc, Chantilly, France). At 2 hours of reperfusion, a midline sternotomy was performed. The right upper lobe of the lung was excised, fixed in 10% formalin, and stained with hematoxylin-eosin. Samples were simultaneously assessed by 2 observers (C.J.S., D.C.W.) who were masked to the experimental manipulation, using a standardized scoring system (Table 1). Pulmonary endothelial leak and edema were assessed by measuring lung wet-dry weight ratios and bronchoalveolar lavage protein content as described previously.23 Myeloperoxidase activity was used as a marker of pulmonary neutrophil infiltration as described elsewhere.24 Protein concentrations and myeloperoxidase activity were measured spectrophotometrically.
A survival study was performed to validate the efficacy of HTS therapy. Lower-torso IR injury was induced in 30 animals as described in the "Model of IR-Induced Lung Injury" subsection. Animals were randomized to receive either isotonic sodium chloride solution (4 mL/kg, n = 10 or 30 mL/kg, n = 10) or HTS (4 mL/kg, n = 10) at 0 and 24 hours' reperfusion via tail vein cannulation. Survival was monitored for 48 hours.
All data are presented as mean ± SEM. A Kruskal-Wallis 1-way analysis of variance on ranks test was used for statistical analysis; P<.05 was considered significant.
Restoration of perfusion resulted in a significant derangement of liver function, represented by marked elevations in plasma levels of the liver enzymes aspartate transaminase and alanine transaminase in all experimental animals compared with controls. The potent immunomodulatory effects of HTS were evident in the attenuation of this acute response in animals resuscitated with HTS (Figure 1).
Significant elevations in the concentrations of the potent proinflammatory cytokines responsible for initiating the acute phase response to IR injury, TNF-α and IL-6, were observed in all animals subjected to lower-torso IR injury; however, this upsurge was attenuated in the HTS pretreatment and treatment groups (Figure 2 and Figure 3). The degree of pulmonary injury sustained as a consequence of IR injury differed markedly between the experimental groups. Tissue samples from the large- and small-volume isotonic sodium chloride solution groups exhibited significant interstitial congestion and hemorrhage, with pronounced derangement of the alveolar architecture. Animals treated and pretreated with HTS evinced significantly lower mean pulmonary injury scores than their isotonic sodium chloride solution counterparts (Figure 4). Increased pulmonary microvascular permeability was detected after IR injury. An escalation in intra-alveolar edema was evidenced by a significant increase in lung wet-dry weight ratio, a response abrogated in the HTS groups but further exaggerated in the large-volume isotonic sodium chloride solution group (Table 2). A marked amplification of transudate protein content, observed in the isotonic sodium chloride solution group, was not mirrored in animals resuscitated with HTS (Table 2).
A significant diminution in pulmonary neutrophil infiltration, confirmed by decreased tissue myeloperoxidase activity, was observed in the HTS-treated and HTS-pretreated groups, contrasting favorably with animals that received isotonic sodium chloride solution (Table 2).
A significant increase in survival was noted in the HTS therapy groups. At 12 hours, survival in the small- and large-volume isotonic sodium chloride solution groups was only 40% and 30%, respectively, whereas animals resuscitated with HTS exhibited 80% survival (Figure 5), a significant increase in survival.
This study demonstrates that HTS resuscitation precludes progression to end-organ injury in states of immunologic disarray arising from IR injury. To our knowledge, no effective intervention strategies currently exist to modulate clinically relevant reperfusion injury and its sequelae. Ventilation is the principal therapeutic maneuver for acute respiratory distress syndrome, although recent interest has focused on suppressing the overexuberant inflammatory response. Attempts to affect the interplay between proinflammatory and anti-inflammatory cytokines may offer the best prospect of diminishing pulmonary involvement, but there are few accepted therapeutic endeavors in this regard.25 Regulators of cellular signal transduction pathways such as p38 mitogen–activated protein kinase and nuclear factor κ β inhibitors provide potential pharmacologic targets for suppressing unchecked and unrestrained inflammation,26,27 although the pharmacodynamics or toxicity of these agents may limit their value in critically ill patients with multiple organ dysfunction syndrome.
Hypertonic saline infusion has been shown to be an effectual means of restoring circulating volume in hemorrhagic shock, exerting favorable effects on cardiac contractility, blood pressure, and peripheral tissue perfusion.28 However, the recognition that HTS infusion can also serve to dampen an overexuberant inflammatory response, and may modulate polymorphonuclear leukocyte cytotoxicity, has revived interest in it as a resuscitative agent. Plasma sodium chloride concentration is tightly regulated, being a major determinant of plasma osmolality. Previous studies have demonstrated that infusion of 7.5% sodium chloride results in a rapid, although transient, rise in plasma sodium levels to 40M above isotonic levels,29 which remain elevated for up to 4 hours.30
The failure of isotonic resuscitation, irrespective of volume, to attenuate the pulmonary inflammatory response subsequent to IR injury further confirms the profound effects of osmotic shock on neutrophil function. Although HTS infusion significantly facilitates microvascular circulation, reducing endothelial cell swelling by establishing a potent osmotic gradient,31 the tendency for a hypertonic environment to disrupt neutrophil-endothelial cell interaction and to induce neutrophil apoptosis19 may account for the amelioration of cell-mediated32,33 lung injury discerned in this study. As dysfunction of the regulatory mechanism of apoptosis of entrapped neutrophils is a pivotal component in the propagation of the massive inflammatory response evident in systemic inflammatory response syndrome,7,34 administration of a proapoptotic hypertonic solution may induce neutrophils to enter the programmed cell death sequence, thereby curbing the tendency toward further alveolar epithelial destruction. This hypothesis is sustained by the finding of markedly diminished myeloperoxidase activity in the lungs of HTS-treated animals. Furthermore, the diminished pulmonary injury observed with osmotic perturbation correlates with decreased endothelial permeability, signifying that HTS exposure also results in a moderated discharge of transudate into the alveolar spaces.
The derangement of liver function observed in the isotonic sodium chloride solution group was not replicated in the HTS groups, a finding in agreement with previous studies.35 This may have a significance that transcends that of hepatic protection, as hepatic amplification of pulmonary inflammation via the immunologic potency of the Kopffer cell has been implicated in the exacerbation of lung injury.14 The correlation with increased survival further emphasizes the importance of diminution of lung involvement in efficacious intervention.
The present study demonstrates a significant inhibition of the cytokine-driven inflammatory response, with exposure to hypertonicity resulting in a marked diminution of serum levels of TNF-α and IL-6. The signaling molecule TNF-α exerts a considerable amplifying effect on the acute inflammatory response, triggering up-regulation of neutrophil β2 integrin expression, and that of the corresponding endothelial ligand, intercellular adhesion molecule-1. This facilitates neutrophil-endothelial interaction and ultimately engenders endothelial hyperpermeability. The predominant role of inflammatory cytokines in promoting neutrophil recruitment, adherence, and extravasation is further emphasized by the observation of a positive correlation between extent of reperfusion injury and serum concentration of IL-6.3 The attenuation of IL-6 expression exhibited by HTS-treated animals further emphasizes the suppression of the inflammatory response provoked by hypertonic resuscitation.
The results of this study affirm the potent immunomodulatory effects of HTS infusion. Hypertonic therapy attenuates neutrophil-mediated end-organ insult by guarding against the deleterious effects of unrestrained neutrophil sequestration and activation and encourages a more benign immunologic profile. A clinical trial of hypertonic resuscitation in hyperinflammatory states is warranted.
Corresponding author and reprints: H. Paul Redmond, BSc, MCh, FRCSI, Department of Academic Surgery, Cork University Hospital, Wilton, Cork, Ireland (e-mail: email@example.com).
Accepted for publication July 27, 2002.
This study was presented at the annual meeting of the Surgical Infection Society (Europe), Madrid, Spain, May 3, 2002.
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