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April 1929


Author Affiliations

From the Surgical Clinic and Laboratory of the Peter Bent Brigham Hospital, Boston.

Arch Surg. 1929;18(4):2008-2018. doi:10.1001/archsurg.1929.01140131112076

There are two methods of studying the histologic structure of any tissue. One is by determining the morphology and specific staining affinities of the component cells in fixed sections. This method of attack has brought fruitful results in the classification of the gliomas.1 The other mode of approach, the direct observation of living cells, has not been used extensively in pathologic investigations, for the securing of cells of most human tissues in a viable condition is a matter of considerable technical difficulty. The methods which are at hand, the technics of supravital staining and of tissue culture, have had only a limited application.

Whether or not these special procedures for the study of living cells will find an increasing sphere of usefulness in laboratories of pathology depends as much on the possibility of their adoption as a routine measure without a prohibitive amount of special training as on a

Bailey, P., and Cushing, H.:  Tumors of the Glioma Group , Philadelphia, J. B. Lippincott Company, 1926.
Kredel, F. E.:  Tissue Culture of Intracranial Tumors ,  Am. J. Path. 4: 337, 1928.
Carrel, A., and Baker, L.:  The Chemical Nature of Substances Required for Cell Multiplication ,  J. Exper. Med. 44:503, 1926.Crossref
Craciun, E. C.:  Heparin-Plasma as Stock-Plasma for Tissue Cultures ,  Bull. Johns Hopkins Hosp. 38:327, 1926.
Carrel, A., and Ebeling, A. H.:  Age and Multiplication of Fibroblasts ,  J. Exper. Med. 34:599, 1921.Crossref
Strangeways, T. S. P.:  The Technique of Tissue Culture in Vitro , Cambridge, W. Heffer and Sons, 1924.
It may be stated that the original purpose of this study, undertaken at Dr. Cushing's request, was to learn whether the primitive medulloblast, composing so large a percentage of the fourth ventricle tumors in childhood, was capable of undergoing differentiation on culture into both spongioblasts and neuroblasts. During the few summer months that the author had to give to the work unfortunately only three medulloblastomas were verified.
Bailey, P., and Cushing, H.:  Medulloblastoma Cerebelli; Common Type of Mid-Cerebellar Glioma of Childhood ,  Arch. Neurol. & Psychiat. 14:192 ( (Aug.) ) 1925.
Globus, J. H., and Strauss, I.:  Spongioblastoma Multiforme ,  Arch. Neurol. & Psychiat. 14:139 ( (Aug.) ) 1925.
Carrel, A.:  The Fundamental Properties of the Fibroblast and the Macrophage, II. The Macrophage ,  J. Exper. Med. 44:285, 1926.
Carrel, A., and Ebeling, A. H.:  The Fundamental Properties of the Fibroblast and the Macrophage: III. The Malignant Fibroblast of Sarcoma 10 of the Crocker Foundation ,  J. Exper. Med. 48:105, 1928.