[Skip to Content]
[Skip to Content Landing]
July 1969

Intra-arterial Perfusion of Pancreatic Juice in the Hind Limb of Dogs: Effects on the Peripheral Vascular Resistance

Author Affiliations

From the departments of surgery (Drs. Yacoub and Howard) and physiology (Dr. Appert), Hahnemann Medical College and Hospital, Philadelphia.

Arch Surg. 1969;99(1):40-46. doi:10.1001/archsurg.1969.01340130042008

It has been shown in this laboratory that the intravenous infusion of pancreatic juice in dogs is followed by temporary arterial hypotension.1,2 The drop in blood pressure is more pronounced and prolonged if the trypsinogen of the pancreatic juice has been enterokinase-activated into trypsin before its infusion. It has been postulated that the trypsin, and perhaps other factors in the pancreatic juice, activate kinin precursors in the serum to produce vasodilatation. Aprotinin (Trasylol), as a trypsin and possibly kallikrein inhibitor, when added to the pancreatic juice prior to intravenous infusion of the mixture, did not significantly ameliorate this hypotensive response. Similarly, heating of the pancreatic juice before its intravenous infusion in order to destroy any enzyme protein activity reduced but did not abolish the hypotensive response.

An ultimate aim of these studies has been to elucidate the cause of hypotension in the patient with severe, acute pancreatitis.

In the

Dimbiloglu, M.E., et al:  Relative Tolerance of the Dog to Intravenous Infusions of Pancreatic Juice ,  Surgery 64:805813 ( (Oct) ) 1968.
Yacoub, R.S., et al:  Systemic Manifestations of Acute Pancreatitis: Effects of the Intravenous Infusion of Pancreatic Juice ,  Arch Surg 99:47-53 ( (July) ) 1969.Crossref
Appert, H.E., et al:  The Disappearance of Intravenously Infused Pancreatic Enzymes ,  Surg Gynec Obstet 127:1281-1287 ( (Dec) ) 1968.
Yacoub, R.S.; Appert, H.E.; and Howard, J.M.:  The Metabolism of Pancreatic Amylase and Lipase Infused Intravenously Into Dogs ,  Arch Surg 99:54-58 ( (July) ) 1969.Crossref
Diniz, C.R., et al:  Micromethod for Determination of Bradykininogen in Blood Plasma ,  Nature 192:1194-1195 ( (Dec) ) 1961.Crossref
Brocklehurst, W.E., and Zeitlin, I.J.:  Determination of Plasmakinin and Kininogen Levels in Man ,  J Physiol 191:417-426 ( (July) ) 1967.
Rocha e Silva, M.; Beraldo, W.T.; and Rosenfeld, G.:  Bradykinin, a Hypotensive and Smooth Muscle Stimulating Factor Released From Plasma Globulin by Snake Venom and Trypsin ,  Amer J Physiol 156:261-273 ( (Feb) ) 1949.
Thal, A.P.; Kobold, E.E.; and Hollenberg, M.J.:  The Release of Vasoactive Substances in Acute Pancreatitis ,  Amer J Surg 105:708-713 ( (June) ) 1963.Crossref
Katz, W., et al:  Trypsin Release, Kinin Production, and Shock: Relationship in Experimental and Human Pancreatitis ,  Arch Surg 89:322-331 ( (Aug) ) 1964.Crossref
Diniz, C.R., et al: " Bradykininogen in Some Experimental Conditions ," in Rocha e Silva, M., and Rothschild, H.A. (eds.):  Third Pharmacological Congress and Soc Bras, Farmacologia e Terapeutica, Experimental (FBFTE) , Sao Paulo, Brazil: Edart Livaria ediora Lta., Rua Cone de Cone de Sarzedas, (August) 1966, pp 15-20.
Blumel, G., and Grozinger, K.H.:  Experimentelle Untersuchungen bei der durch Fettemulsionen ausgelösten Pankreatitis der Katze ,  Wien Klin Wschr 78:847-853 ( (Dec) ) 1966.
Ryan, J.W., Moffat, J.G., and Thompson, A.G.:  Role of Bradykinin System in Acute Hemorrhagic Pancreatitis ,  Arch Surg 91:14-24 ( (July) ) 1965.Crossref
Cannon, R.H., and Turner, M.D.:  Retardation of the Progress of Experimental Pancreatitis With Trypsin Inhibitors ,  Surg Forum 12:367-369 ( (Oct) ) 1961.
Ribacoff, S., et al:  Study of the Effect of Trypsin Inhibitor in Acute Hemorrhagic Pancreatitis in Dogs ,  Surg Forum 12:370-372 ( (Oct) ) 1961.
Kelly, T.R.; Bratcher, E.P.; and Falor, W.H.:  Trypsin Inhibitor in Acute Hemorrhagic Pancreatitis in Dogs: Effect ,  Arch Surg 89:317-321 ( (Aug) ) 1964.Crossref
Nugent, W.F., et al:  Early Experience With Trasylol in the Treatment of Acute Pancreatitis ,  Southern Med J 57: 1317-1321 ( (Nov) ) 1964.Crossref
Nugent, W.F., and Atendido, W.A.:  The Role of Antienzymes in the Treatment of Acute Pancreatitis ,  Amer J Gastroent 43:437-442 ( (May) ) 1965.
Singh, L.M., and Howard, J.M.:  Studies of Trasylol: Evaluation of Trasylol Against the Hypotensive Effects of Trypsin and Kallikrein ,  Arch Surg 91:635-639 ( (Oct) ) 1965.Crossref
Forell, M.M.:  Therapy with Kallikrein and Protease Inhibitors ,  Ann NY Acad Sci 104:368-375 ( (Feb) ) 1963.Crossref
Erdos, E.G., and Sloane, E.M.:  An Enzyme in Human Blood Plasma That Inactivates Bradykinin and Kallidins, Biochem  Pharmacol 11:585-592 ( (July) ) 1962.
Erdos, E.G., and Westerman, M.P.:  Properties of a Carboxypeptidase in Blood Sera ,  Fed Proc 22:425 ( (April) ) 1963.
Erdos, E.G.; Whaler, J.R.; and Levine, M.I.:  Blocking of the In Vivo Effects of Bradykinin and Kallidin With Carboxypeptidase-B ,  J Pharmacol Exp Ther 142: 327-334 ( (Dec) ) 1963.